摘要
目的建立豚鼠至大鼠非协调性异种肝移植动物模型,并对动脉化模型和静脉化模型进行比较。方法共进行异种肝移植40次,其中20对为动脉化组,20对为静脉化组。比较两组存活时间、受体血中肝脏酶的变化,移植肝组织学改变和荧光抗体染色。结果动脉化组存活时间为(92.95±28.52)min,静脉化组为(135.10±46.12)min。两组移植肝组织学变化基本相似,肝细胞水样变形、血管和血窦淤血;荧光染色见 IgM 和 IgG 沉积于血管内皮细胞和肝血窦。结论在没有克服超急性排斥反应之前,豚鼠至大鼠异种肝移植静脉化模型比动脉化模型更简单实用。
Objective To establish the model of guinea pig-to-rat discordant hepatic xenotransplantation and make a comparison between the model with graft rearterialization and that without.Methods A total of 40 pairs of hepatic xenotransplantation were done.Of these 40,20 were rearterialized and the other 20 were not.Sur- vival time,variation of liver enzyme,histology of the grafts and fluorescent deposition of IgM and IgG were deter- mined in both groups.Results The survival time was shorter in the rearterialized group than that in the nonarteri- alized group.The same histological changes of diffuse liver cell swelling, blood stasis in the vessels and liver sinus were seen in both groups.Fluorescent dying showed that IgM and IgG were deposited in the vascular endothelium and liver sinus.Conclusions In the hepatic xenotransplantation from guinea pig to rat,the nonrearterialized mod- el is simpler and more practical than the rearterialized one before controlling of hyperacute rejection.
出处
《中华肝胆外科杂志》
CAS
CSCD
2001年第7期422-424,共3页
Chinese Journal of Hepatobiliary Surgery
基金
上海市科委资助项目(98XD14002)
