摘要
目的 探讨正常胃粘膜、胃粘膜肠化生组织及胃癌组织中TGF βⅡ的表达及细胞凋亡情况。 方法 采用免疫组化法检测其TGF βⅡ表达 ,采用DNA原位末端标记法 (TUNEL)检测其细胞凋亡。 结果 正常胃粘膜、肠化生组织及胃癌的细胞凋亡指数(% )分别为 1 72 7± 1 5 93、5 16 1± 4 6 73、1 6 2 2± 2 46 9;TGF βⅡ阳性率分别为 10 % (1/10 )、2 9 5 % (13/4 4 )、73 6 % (31/4 2 )。 结论 由正常胃粘膜、肠化生到胃癌TGF βⅡ表达率逐渐上升 ,而细胞凋亡指数则肠化组织中最高 ,胃癌组织中较低 ,提示TGF βⅡ。
Aim To investigate the expression of TGF βⅡ and apoptosis in normal epithelial cells,intestinal metaplasia of gastric mucosa and gastric carcinoma.Methods TUNEL method was used to detect apoptosis,and mmunohistochemical analysis was used to detect the exepression of TGF βⅡ.Results In the normal epithelial cells,intestinal metaplasia of gastric mucosa and gastric carcinoma,apoptotic indexes(%) were 1 727±1 593,5 161±4 673,1 622±2 469.The positive rate of TGF βⅡ was 10%(1/10),29 5%(13/44),and 73 6%(31/42) respectively.ConclusionS The results suggest that,during the changes of gastric carcinoma,the expressive rate of TGF βⅡ gradually increased from the normal epithelial cells to intestinal metaplasia of gastric mucosa and gastric carcinoma,the apoptotic indexes were highest in intestinal metaplasia of gastric mucosa.The exepression of TGF βⅡ and apoptosis may play an important role in the genesis and developmant of gastric carcinoma.
出处
《胃肠病学和肝病学杂志》
CAS
2001年第2期168-169,113,共3页
Chinese Journal of Gastroenterology and Hepatology
关键词
胃癌
肠化生
TGF-βⅡ
细胞凋亡
病理
Gastric carcinoma Intestinal metaplasia of gastric mucosa TGF βⅡ Apoptosis
