摘要
目的:研究甲基莲心碱对人乳腺癌细胞阿霉素耐药株MCF7/Adr多药耐药multidrugresistanceMDR性的逆转作用及其机制。方法:采用MTT法检测药物细胞毒性作用,高效液相色谱法检测细胞内阿霉素ADR的浓度,流式细胞术测定细胞P糖蛋白Pgp的表达。结果:甲基莲心碱1～10μmol·L-1能不同程度地降低ADR对MCF7/Adr细胞的IC50。10μmol·L-1甲基莲心碱能显著提高ADR在MCF7/Adr细胞内的浓度,降低MCF7/Adr细胞Pgp的表达。结论:甲基莲心碱能逆转MCF7/Adr细胞的MDR,其机理与抑制Pgp的功能及其表达,增加细胞内ADR的积累有关。
Objective: To investigate the reversal effect of neferine(Nef) on adriamycin resistant human breast cancer cell line(MCF 7/Adr) and its mechanisms. Methods: The cytotoxic effect of chemotherapeutic agents was determined by MTT assay. The intracellular adriamycin(ADR) concentration was assayed by HPLC. The expression of P glycoprotein (P gp) was examined by flow cytometric(FCM) assay. Results: Nef (1-10 μmol·L-1) decreased the IC50 of ADR in MCF 7/Adr cells. 10 μmol·L-1 Nef significantly increased the intracellular concentration of ADR and reduced the expression of P gp in MCF 7/Adr cells. Conclusions: Nef can reverse Multidrug resistance(MDR) of MCF 7/Adr cells. Its reversal mechanisms were associated with the increase of the intracellular accumulation of ADR resulted from decrease of P gp pump efflux activity and the expression of P gp in MCF 7/Adr cells.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2001年第8期831-833,共3页
Chinese Journal of Cancer
基金
湖南省教委科研基金项目NO:96155
