摘要
目的 :探讨双侧海马细胞癫痫相关性单位放电特征。方法 :双玻璃微电极同步记录大鼠 44对双侧海马神经元单位放电 ,每隔 5～ 10min重复强直电刺激右背海马 (0 .6～ 0 .4mA 60Hz 2s)一次 ,共施加 10～ 12个刺激串。结果 :强直电刺激可以诱发双侧海马神经元单位放电的原发性和继发性后放 ,呈现明显的双侧非对称性和动态发展特征 ,甚至出现双侧交互性改变 ,与人类颞叶癫痫的病理生理特征相吻合 :进行性发展、跨大脑半球扩布和动态变化。强直电刺激对海马细胞单位自发放电具有易化或抑制、调制或解调作用 ,并取决于这些细胞的基础单位放电。东莨菪碱可以解调电刺激引起的海马细胞爆发式单位放电成为紧张性放电 ,诱导强直电刺激后单位放电频率的抑制效应。结论 :强直电刺激右背海马后 。
Aim: To study the characteristics of unit discharges related to epilepsy of hippocampal neuron in both hemispheres in intact rats. Methods: 44 pairs of cells were extracellularly recorded with dual glass microelectrodes from bilateral hippocampi before or after administration of repetitive tetani (0.4～0.6 mA, 2 s, 60 Hz) to the right dorsal hippocampus. Repeated tetani were used about ten times at 5 or 10 min intervals to ensure full expression of afterdischarges without the tissue being in postictal refractory period. Results:Primary or secondary unit afterdischarges of hippocampal neurons were evoked by tetani. They were characterized by bilateral asymmetry, moveability and interconvertion between two hemispheres, which were observed in temporal lobe epileptic humans. In addition facilitatory or inhibitory, modulating or demodulating effect of tetani on spontaneous unit discharges depended on the basic firing rates or patterns of hippocampal cells before stimulation. Rhythmic cell bursting of the hippocampus was demodulated to tonic firing by Scopolamine (0.05 mg/kg, i.p.). Tetanus induced inhibition of hippocampal unit discharges was observed after administration of scopolamine. Conclusion: Abnormal electrophysiological activity of bilateral hippocampi neurons evoked by tetanus may be the pathophysiological bases for dual hemispherical sclerosis and atrophy in temporal lobe epilepsy.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2001年第3期262-266,共5页
Chinese Journal of Applied Physiology
基金
湖北省自然科学基金资助项目 (99J13 7)
