摘要
目的 研究异体复合组织移植 手移植术后病人外周血T淋巴细胞亚群及CD3+ HLA DR +T细水平的动态变化。方法 用流式细胞术测定了 2例异体手移植术后不同时间外周血CD3+ 、CD3+ CD4+ 、CD3+ CD8+ 、CD3+ HLA DR+ (活化T细胞 )细胞百分率 ,及CD4/CD8比值 ,以病人术前一周结果作对照。结果 使用免疫抑制剂后 ,于术后第 1日CD3+ 、CD3+ CD4+ 、CD3+CD8+ 、CD3+ HLA DR+ 细胞水平 ,CD4/CD8比值都开始降低 ,以 3～ 5日为最低水平。术后 8日 ,上述指标逐渐回升 ,至 15日后基本趋于平稳 ,但CD3+ 、CD3+ CD4+ 细胞及CD4/CD8比值仍明显低于术前 ,而CD3+ CD8+ 、CD3+ HLA DR+ 细胞水平则高于术前。结论 异体复合组织 手移植术后病人外周血T淋巴细胞亚群及CD3+ HLA DR+ 细胞水平的变化与单一组织器官移植 (肾移植 )术后稳定期的变化一致 ,也与病人术后的稳定病情相吻合。
Objective\ To study dynamic changes of T cell subsets and CD3 +HLA DR +T cells in peripheral blood of composite tissue transplantation hand allograft recipients. Methods \ The levels of CD3 +、CD3 +CD4 +、CD3 +CD8 +、CD3 +HLA DR +T cells and ratio of CD4/CD8 in peripheral blood of hand allograft recipients in different periods were examined by using flow cytometry.The recipients before transplantation were as the control groups.Results\ The first day after transplantation,the levels of CD3 +、CD3 +CD4 +、CD3 +CD8 +、CD3 +HLA DR +T cells and ratio of CD4/CD8 all began to descend.The 3th to 5th day after transplantation,the levels were the lowest.The 8th day,the levels of CD3 +、CD3 +CD4 +、CD3 +CD8 +、CD3 +HLA DR +T cells and ratio of CD4/CD8 were eventually rised and go to stable 15th day after transplantation.But the levels of CD3 +、CD3 +CD4 +and value of CD4/CD8 were still lower than those of the contols,and the levels of CD3 +CD8 +、CD3 +HLA DR +T cells were higher distinctly.Conclusion\ Dynamic changes of T cell subsets and active T cells in peripheral blood of composite tissue transplantation hand allograft recipients were accordant with that of renal allograft recipients with stable period and with stable clinical state of the hand transplantation recipients.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2001年第3期155-156,共2页
Chinese Journal of Clinical Laboratory Science
