摘要
目的 研究 RER阳性与 RER阴性脑胶质瘤中 TGF- β R基因突变的差异 ,探讨 TGF- β R基因突变在胶质瘤发生中的作用。方法 运用 PCR- MIA、PCR- SSCP、PCR-直接测序技术测定 40例脑神经胶质瘤手术切除标本中 ,复制错误阳性组 (RER阳性 )与 RER阴性组之间 TGF-β R基因突变的差异。结果 原发性脑胶质瘤 40例中 8例 (2 0 % )表现为 TGF-β R基因异常。其中 RER阳性 8例中 6例 (75.0 % ) ,RER阴性 32例中 2例 (6.3% )表现 TGF- β R基因异常 ,二者有显著差异 (P=0 .0 0 0 2 )。结论 TGF- β R基因突变在 RER阳性星形细胞瘤中发生率较高 ,TGF-β R基因异常可能是 、
? Objective To research the gene mutation difference of TGF β between RER positive glioma and RER negative cases,to evaluate the role of TGF β ⅡR gene mutation on the pathogenesis of glioma.Methods To determine the gene mutation difference using PCR MIA?PCR SSCP and PCR sequencing techniques.Results (1)8 cases were RER positive out of 10 cases with microsatellite instability;(2)Out of all 8 RER positive cases,6 cases showed TGF β ⅡR abnormal changes(75 0%),But only 2 cases out of 32 RER negative cases showed TGF β ⅡR abnormal changes(6 3%).The difference between the two groups was significant( P =0 0002)?Conclusions TGF β ⅡR abnormal changes may play an important role in the pathogenesis of high grade of glioma as RER positive is often associated with Ⅲ?Ⅳ grade astrocytoma.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2001年第4期254-255,共2页
Chinese Journal of Gerontology
