摘要
于 1 998年 3月在青岛市太平角采集裙带菜孢子体 ,通过破碎、离心制备类囊体膜。用去污剂SDS、DOC、DMG和DIG增溶裙带菜类囊体膜 ,经PAGE分离色素 -蛋白质复合物 ,并测定其光谱特性。结果表明 ,电泳分离效果与所用的去污剂的种类有关。去污剂不同 ,分离到的同一类型复合物的吸收光谱有差异 ,捕光色素 -蛋白质复合物的数目、种类及吸收光谱明显不同。电泳中产生的游离色素的多少也随去污剂种类而异。在 4种去污剂中 ,以DMG和DIG增溶的效果最好 ,经SDS PAGE分出 6种色素 -蛋白质复合物 ,游离色素较少。
The thylakiod membranes isolated from a brown alga Undaria pinnatifida were solubilized by detergents sodium dodecyl sulfate (SDS), deoxycholate (DOC), decanoyl-N-methylglucamide (DMG) and digitonin (DIG) at different concentrations (SDS∶Chl=10∶1,DOC∶Chl=60∶1,DMG∶Chl=20∶1,DIG∶Chl=50∶1). The pigment-protein complexes were separated by the SDS-PAGE, and their spectrum characteristics were determined. The results of PAGE separation varied with detergents. DOC solubilization yielded 10 pigment-containing bands and resulted in a large number of free pigments. SDS solubilization yielded only 5 pigment-containing bands and lots of free pigment were produced. Comparing with SDS and DOC, DMG and DIG solubilization resulted in 7 pigment-containing bands and only a little free pigment. When thylakoid membranes were solubilized by different kinds of detergents and separated by SDS-PAGE, the same pigment-protein complexes had different absorption and fluorescence emission spectra. The absorption maxima of chlorophyll-a in PSI complexes were different in blue and red lights, and its short-wavelength fluorescence emission maxima at 77K also varied with detergent. PSII complexes treated with DMG or DIG had absorption of fucoxinthin. The light-harvesting pigment-protein complexes (LHC) were also different in number, type and absorption spectrum. Two Chl a/c-protein complexes were resolved by SDS and four by DOC, but no fucoxinthin-Chl a/c-protein complexes (FCPC) were separated by SDS or DOC. DMG or DIG solubilization can yield three types of LHC, of which were two FCPC and one Chl a/c-protein complexes. According to the PAGE results, the best separation was obtained by using DMG or DIG, in which 6 pigment-protein complexes were resolved and a little free pigment were produced.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2001年第4期394-401,共8页
Oceanologia Et Limnologia Sinica
基金
国家"九五"攻关资助项目96-C0 1 -0 5-0 1号
关键词
裙带菜
去污剂
凝胶电泳
色素-蛋白质复合物
吸收光谱
荧光光谱
分离效果
Undaria pinnatifida, Detergent, SDS-PAGE, Pigment-protein complexes, Absorption spectra, Fluorescence emissions spectra
