摘要
目的 :研究负电晕充电的聚四氟乙烯 (PTFE)驻极体对成纤维细胞凋亡的影响及其调控机制。 方法 :选用 - 30 0、- 5 0 0和 - 10 0 0 V PTFE驻极体作用于成纤维细胞 2 4、48和 72 h,利用流式细胞仪和透射电子显微镜研究负极性驻极体对成纤维细胞凋亡的影响。结果 :- 30 0、- 5 0 0和 - 10 0 0 V驻极体作用成纤维细胞 2 4、48和 72 h以后 ,与对照组相比 ,成纤维细胞的凋亡量从 0 .5 %增至 10 % (部分可达 15 % ) ;驻极体作用成纤维细胞 48～ 72 h,出现细胞凋亡特有的形态学特征 ,即 :细胞异染色质边集 ,细胞裂解 ,可见凋亡小体。驻极体诱导成纤维细胞凋亡的效应与作用时间和场强呈正相关性 (P<0 .0 5 )。 结论 :负极性驻极体有促进成纤维细胞凋亡的作用。
Objective: To study the influence of negative electrets on apoptosis of fibroblast cells and to probe its mechanism. Methods: Fibroblast cell were treated with -300, -500 and -1 000V PTFE electrets for 24, 48 and 72 h, respectively, and the influence of negative electrets on cell apoptosis was studied by means of flow cytometry and transmission electron microscope. Results: Compared with control group, apoptosis cells increased from 0.5% to 10% (some even to 15%) after 24,48 and 72 h action of -300, -500 and -1 000 V electrets. After action of -500 V PTFE electrets for 48 72 h, fibroblast cells showed characteristic morphological features of apoptosis. These features included chromatin aggregation, nuclear and cytoplasmic condensation and partition of cytoplasm and nucleus into membrane bound vesicles (apoptotic bodies). The effect of negative electrets on apoptosis was in proportion to the time and electric field intensity. Conclusion: Negative electrets can enhance apoptosis of fibroblast cells.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2001年第5期409-411,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金资助项目 ( 3 9770 3 69)
