摘要
用WizardDNAClean upSystem纯化葡萄抗霜霉病基因RAPD遗传标记OPO 0 6 - 150 0片段 ,用T easyVector克隆RAPD标记 ,采用自动荧光DNA测序仪 (型号ABI 377DNASe quencer)测定了该DNA片段的核苷酸组成及其排列顺序 .来自中国野葡萄的RAPD标记OPO 0 6- 150 0从左右两端各测了 586bp和 4 52bp .对两端序列的酶切位点进行了分析 .根据两端序列可以设计专一PCR扩增引物作为合成抗霜霉病检测探针的基础 ,用于检测葡萄抗病育种和抗病品种的霜霉病抗性 .
This paper presents the sequence of RAPD marker linked to the downy mildew resistance genes in grapes.The isolation DNA was purified by Wizard DNA Clean up System and inserted into plasmid pGEM T easy vector.The sequencing was performed based upon automated fluorescence DNA sequencer(Model ABI 377).The marker OPO 06-1 500 from the resistant wild grape native to China consists of about 1 500 bp. 586 bp and 452 bp of OPO 06-1 500 were sequenced from two directions,and their restriction sites were analyzed by using PC gene software.It is suggested that the 5′ end 3′ end sequences of OPO 06-1 500 might be used as basis for finishing the total sequence and synthesizing the specific PCR primers and the probe for detecting grape downy mildew resistance both in disease resistant breeding and identification of disease resistant varieties from it.
出处
《海南大学学报（自然科学版）》
CAS
2001年第2期146-150,共5页
Natural Science Journal of Hainan University
基金
热带作物生物技术国家重点实验室开放基金的资助
