摘要
利用脂质体转染法将大鼠 4.5SsnRNAcDNA重组载体转染小鼠肾离体培养细胞 ,连续培养 48h、96h和 144h检测荧光素酶活性发现 ,4.5SsnRNAcDNA插入表达载体的方向不同对荧光素酶基因表达的影响无差异 ;重组体转化的细胞中荧光素酶基因表达的活性与对照组相比提高 2 - 2 .2倍 ;4.5SsnRNAcD NA对荧光素酶基因表达的影响 ,随转染细胞培养时间的延长而表达活性迅速下降 ,其下降速度实验组比对照组要快得多 .
The recombinant plasmids pSVluc20-4.5s(+) and pSVluc20-4.5s(-) were transferred into mouse’s kidney cultural cells by liposome transfection experiments . These transfered cells were continually cultured 48h, 96h and 144h, and then the cellular luciferase activity were detected . The results showed that after 4.5S cDNA sequence was inserted into the vectors by different direction, no difference was produced in the luciferase gene expression. As these vectors were transferred into cultural cells, the activity of luciferase was 2-2.2 times as mush as the contrast. In addition, there was a marked drop in the activity of luciferase as the culture time of transferred cells were prolonged. Falling speed of enzyme activity in the experiment groups was larger than the contrasts. Through analysing 4.5S cDNA sequences, its molecular structure contains the characters of enhancer. It is suggested that 4.5S snRNA cDNA have possibly enhancering effect in the gene regulation.
出处
《曲阜师范大学学报（自然科学版）》
CAS
2001年第3期77-80,107,共5页
Journal of Qufu Normal University(Natural Science)
基金
国家自然科学基金项目 (39970 10 3)
中国博士后基金项目资助
