摘要
目的：探讨前列腺癌患者骨髓中微转移癌细胞的检测方法。方法：术前采集前列腺癌５９例的骨髓标本并收集相关临床资料，应用细胞角蛋白的免疫细胞化学方法进行检测，并将结果与ＴＮＭ Ｇ分期（级）进行相关分析。结果：骨髓中ＣＫ＋细胞检出率为２５．４％，除Ｇ３／４组阳性率（４５．５％）明显高于Ｇ１／２组（１２．５％，Ｐ＝０．０１）外，其余各组间的阳性率，差异均无显著性意义。结论：免疫细胞化学方法可作为检测骨髓中隐蔽的微转移肿瘤细胞的一种敏感的标准方法，肿瘤细胞的分化程度可能与其离开原发灶发生播散的能力有关，该指标是一个独立的危险因素，对预后评估和治疗方法的选择与确定有指导意义。
Purpose:To detect individual tumour cells of micrometastases in the bone marrow of patients with prolate cancer. Methods :Preoperative bone marrow was aspirated in all 59 patients with prostate cancer(PC). An immunocytochemical cytokeratins(CK) assay was used to allow the identification of individual tumour cells disseminated to bone marrow and the analysis of relation between the incidence of tumor cell detection and the TNM classification and tumor differentiation grading was carried. Result: CK+ cells in the bone marrow were detected in 25. 4 % of the patients with PC;The positive rates of G3/4 patients with PC(45. 5%) was significantly higher than those(12.5%) of G1/2 patients( P =0.01) No statistically significant differences were found in positive rates between the rest of another group. Conclusions: An immunocytochemical staining assay for cytokeratins must be extremely sensitive standard method of micrometastic detection. Differentiation of tumour cells may be related to dissemination. Micrometastases in the bone marrow is an independent risk factor and could have a substantial clinical impact on the prognosis and optimal therapy for patients with cancer.
出处
《临床泌尿外科杂志》
2001年第5期222-224,共3页
Journal of Clinical Urology
