摘要
以徐州１４２及其无绒无絮（无纤维）突变体（ｆ１）为材料，利用ＡＦＬＰ标记技术对该近等基 因系进行ＤＮＡ多态性分析。在６４对引物产生的６ ３６０多条扩增带中，发现１条差异带（ＣＦ１） 稳定出现在有纤维和无纤维之间。进一步用徐州１４２无线无絮突变系与纤维发育正常的渝棉一号的杂交后代 Ｆ２、Ｆ３分离群体以及几个陆地棉品种作材料验证，结果表明，差异带（ＣＦ１）与 纤维的产生呈共分离，该差异带是与棉花纤维发生相关的分子标记。进而对 ＣＦ１进行回收、克 隆和序列测定，ＣＦ１长２０５ｈｐ，通过６个开放阅读框推断的氨基酸序列，与酚羟化酶ａ－亚基、外 表皮蛋白Ｃ、ＮＡＤＨ脱氢酶亚基１、ＮＡＤＨ－ＣｏＱ氧化还原酶、２－氧代－铁氧还蛋白－氧化还原酶 以及假拟 １４．５ｋＤ蛋白（ｈｙｐｏｔｈｅｔｉｃａｌ １４．５ｋＤ ｐｒｏｔｅｉｎ）有较高的相似性。
By using AFLP technique polymorphism analysis was performed between a fuzzlesslintless mutant line and its isogenic wild-type line, Xuzhou 142. Out of 6 360 bands produced by 64 pairs primers, a fragment named as CF1, appearing stably in wild-type line, Xuzhou 142. This polymorphism was further verified using several normal fiber varieties and F2, F3 populations from the cross of fuzzless-lintless mutant line with a high-lint-percentage variety Yumian No. 1. The cosegregation of CF1 and fibrogenesis was proved, which suggested that CF1 can be used as a molecular marker for cotton fibrogenesis. The CF1 segment was cloned into PUCm-T Vector and then sequenced. The putative amino acid sequences, is an analogue to phenol hydroxylase alpha subunit outer surface protein C, NADH dehydrogenase subunit 1, NADH- ubiquinone oxidoreductase, 2-oxoacid ferredoxin oxidoreduct and hypothetical 14.5kD protein.
基金
国家自然科学基金重点资助项目(批准号:39830240)
国家教委博士点基金(批准号:1999-006003)
