摘要
亚克隆测序分析发现 ,在 Bradyrhizobium japonicum的 GX2 0 1菌株染色体的 1 6kb Eco R DNA区段上含有一个与 put A基因同源的基因的 ORF30 54( Open Reading Frame) .该基因 ORF长 30 54bp,在核苷酸水平上与已报道的 put A基因有 94 %一致性 .其推断性的编码产物在氨基酸水平上与 put A编码产物脯氨酸脱氢酶 ( Pro DH)有 95%一致性 .利用 Tn5gus A5诱变的方法获得了该同源基因的标记置换突变体 ,该突变体在液体培养基 ( YMA)
The results of the subcloning and sequencing analysis showed that there is a perfect ORF3054 (Open Reading Frame) homologus with the put A gene in the 16kb Eco RⅠ DNA fragment cloned from the chromosome of Bradyrhizobium japonicum GX201,the length of which was 3054 base pairs. It shared 94% identity with the DNA of the put A gene at the nucleotide level, and its putative product protein showed 95% identity with the proline dehydrogenase (ProDH) encoded by put A gene at amino acid level. When cultured in the liquid medium YMA,the growth ratio of the markerexchanger mutant of this homologous gene was lower than that of the wildtype strain.
出处
《广西大学学报（自然科学版）》
CAS
CSCD
2001年第2期150-155,158,共7页
Journal of Guangxi University（Natural Science Edition）
基金
国家高技术"863"计划! ( 863 1 0 1 0 3 0 3 0 1 )
