摘要
目的 :研究糖尿病心肌的病理变化及其发生机制。方法 :用光镜及透射电镜观察四氧嘧啶诱导的糖尿病 1个月大鼠心肌形态学改变 ,并测定心肌组织超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)、一氧化氮合酶 (NOS)的活性及一氧化氮 (NO)、丙二醛 (MDA)含量。结果 :光镜下见心肌细胞萎缩、嗜酸性变及空泡变性 ,间质纤维增生 ,透射电镜下见线粒体扩张、嵴变短 ,内质网扩张 ,肌原纤维破坏 ,间质胶原纤维增生。SOD、GSH Px活性下降 ,NOS活性及NO、MDA含量增加。结论 :糖尿病大鼠心肌病变主要为心肌萎缩、线粒体扩张及肌原纤维破坏 ,间质纤维增生 。
Purpose To study myocardial pathology and its pathogenesis in diabetes. Methods Myocardial structure of alloxan induced diabetic rats was observed under light microscopy (LM)and transmission electron microscopy(TEM).Activity of superoxide dismutase(SOD),glutathione peroxidase (GSH Px),nitric oxide synthase(NOS)and content of malondialdehyde (MDA),nitric oxide(NO) were detected biochemically in myocardial homogenate. Results Atrophy and degeneration of myocardium and interstitial fibrosis were found under LM and expansion of mitochondria and smooth endoplasmic reticulum, destruction of myofibril and interstitial proliferation of collogen fiber under TEM. Activity of SOD and GSH Px decreased significantly, while content of NO and MDA and activity of NOS increased significantly in diabetic rats. Conclusion Atrophy of myocardium, expansion of mitochondria,destruction of myofibril and interstitial fibrosis are the main morphological changes of diabetic cardiomyopathy, and lipid peroxidation and NO may be involved in it.
出处
《临床与实验病理学杂志》
CAS
CSCD
2001年第2期146-148,共3页
Chinese Journal of Clinical and Experimental Pathology
基金
浙江省教育厅资助课题!(No 2 0 0 0 0 6 74)
温州市 5 5 1人才基金专项资助课题
关键词
实验性糖尿病
心肌
过氧化脂质类
一氧化氮
大鼠
diabetes mellitus, experimental
myocardium
lipid peroxides
nitric oxide
rats
