摘要
目的 探讨脐血与成人外周血中淋巴细胞亚群分布及细胞因子的差异及其与移植物抗宿主病 (GVHD)发生的关系。方法 采用流式细胞术、三色荧光单克隆抗体标记对脐血和成人外周血中淋巴细胞进行分类 ,并对其分泌的IL 2、TNF α和IFN γ进行分析比较。结果 ①脐血中T淋巴细胞以CD4 + CD4 5RA+ 细胞为主 ,而成人外周血以CD4 + CD4 5RO+ 和CD8+ CD4 5RO+ 两种表型为主 ;②脐血淋巴细胞产生IL 2、TNF α和IFN γ的量明显低于成人外周血淋巴细胞。进一步分析表明 ,脐血中产生细胞因子的细胞大部分为CD4 + CD4 5RA+ 细胞 ,而成人外周血中大部分为CD4 + CD4 5RO+ 和CD8+ CD4 5RO+ 细胞。结论 成人外周血与脐血淋巴细胞不仅存在亚群比例上的差异 ,而且各亚群分泌细胞因子的量也明显减少。
Objective To explore the difference of intracellular cytokines between cord blood and adult peripheral blood and elucidate its significance for GVHD. Method Different lymphocyte subsets were categorized in cord and adult peripheral blood by monoclonal antibodies with 3 color fluorescence using flow cytometry. The IL 2, TNF α and IFN γ produced by various lymphocyte subsets were compared between the two groups. Result ①In cord blood, dominant T cells were CD 4 +CD 45RA +, while the major T cells were both CD 4 +CD 45RO + and CD 8 +CD 45RO + in adult peripheral blood . ②The quantities of IL 2, TNF α and IFN γ produced by cord blood lymphocytes were lower than that of adult peripheral blood lymphocytes. Moreover, the majority of cells that produced cytokines in the cord blood were CD 4 +CD 45RA +, but in adult peripheral blood were mainly CD 4 +CD 45RO + and CD 8 +CD 45RO +. Conclusion As compared with adult peripheral blood, the cord blood not only possessed different proportion of lymphocyte subsets, but also significant low level of cytokines. The differences of various lymphocyte subset proportions and lower level of cytokines in cord blood probably related to the lower incidence of GVHD in cord blood stem cell transplantation.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2001年第6期288-291,共4页
Chinese Journal of Hematology
关键词
胎血
外周血
淋巴细胞
细胞因子
移植物抗宿主病
Cord blood
Peripheral blood
Lymphocyte
Cytokine
Graft versus host disease
