摘要
将CB－HRP经电泳引入火鸡左侧古纹状体，动物分别存活2h、5h、12h、17．5h、24h、48h，灌杀。做脑干和大脑半球的冰冻连续切片，TMB成色，中性红复染，光镜下明、晴视野对照观察。结果发现：1．CB－HRP被引入古纹状体后，其酶液扩散范围于2h最大直径为1．689mm；5h达到2.675mm;8h亦达2.675mm，12h时为2．253mm；17．5h时为1．830mm；24h时为0.845mm;48h时为0.422mm.2．CB－HRP被引入12h时在耳蜗居核、顶盖灰质及三叉神经感觉主核出现小范围的顺行标记给本，及至48h时脑干内出现广泛的颁行标记物。经测算其顺行运输速率可达96mm／d。
This paper dealed with the valid diffusion area s'size and the antigridetransported speed of the CB- HRP, as a neuroanatomical tracer,that was applied intothe archistriatalum of the left telecepholon by means of the iontophoresis in chicken.The iontophoretical current was managed on +4μA, 7 seconds on, 7 seconds off,and thetotal time was 30 minutes. After application of the CB-HRP,the animals recieved various survive times of 2 hours, 5 hours, 8 hours, 12hours, 17. 5 hours 24 hours, and 48hours. The results are as following: 1. The available area of the iontophouesis wasbiggest at the 5 hour survive time and 8 hour survive time and the diameter was 2.675mm. 2. The antigride transport rate was at least 96mm/day.
基金
内蒙古自然科学基金
