摘要
目的 :建立用气 -质联用仪测定抗焦虑新药 (AF - 5 )的血药浓度的分离分析方法 ,并对AF - 5不同剂型的血药浓度进行测定。方法 :血浆样品经正己烷一石油醚 (9∶1)提取 ,以高纯氦为载气 ,DB - 5MS毛细管柱 ,正二十一烷为内标进行定性定量分析。结果 :检测限为 0 0 5 6ng (S N =6 ) ,最低检测浓度为 8 5ng·mL- 1 血浆。AF - 5的血药浓度在 8 5～ 110 0ng·mL- 1 范围内线性关系良好。回收率为 81 6 %。结论 :该方法操作简便 ,特异性强 ,重现性好 ,适用于药代动力学研究和血药浓度监测。
Objective:To establish a GC-MS method for the separation and determination of a novel anti-anxietic drug(AF-5)and to determine the plasma concentration of AF-5 administered in different preparations.Methods:Plasma samples were extracted with n-hexane-petroleum ether(9∶1)and analyzed qualitatively and quantitatively on DB-5MS capillary column using n-henicosane as the internal standard and high purity helium as the carries gas.Results:The limit of detection is 0.056 ng(S/N=6)the limit of detectable concentration is 8.5 ng ·mL -1plasma.There is a good linear relationship between the plasma AF-5 concentration and response in the range of 8.5～1100 ng·mL -1.The recovery is 81.6%.Conclusion:The method developed is easy to operate,of good specificity and reproducibility.It is suitable for pharmacokinetic studies and monitoring plasma concentration.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2001年第3期176-179,共4页
Chinese Journal of Pharmaceutical Analysis
