摘要
针对非洲菊自然繁殖率低下的情况 ,探讨了利用组织培养方法解决生产中繁殖的问题 .结果显示 :在茎段、茎尖和叶柄 3种外植体中 ,茎尖的出愈率最高 ,是理想的快速繁殖材料 ,较适宜的诱导愈伤组织的培养基为MS +BA 1.0mg·L-1+IBA 0 .0 5mg·L-1+庶糖 3.0 % ,诱导不定芽的培养基为MS +BA 2 .0mg·L-1+IAA 0 .5mg·L-1+庶糖 3.0 %或MS +Kt3.0 +IAA0 .0 5mg·L-1+庶糖 3.0 % ,而根的诱导则是在MS +NAA 0 .3mg·L-1+IBA 0 .3mg·L-1+庶糖 3.0
Because the fruiting rate and germinative percentage of Gerbera jamesonii Bolus are lower, tissue culture was explored to solve the propagating problem in the production. The results show that the shoot-tip is the best material in speeding propagation among the three explants (shoot-tip, stem and petiole) as it has the biggest inductivity, the best growth and it is easy to be inducted adventitious buds. The suitable phytohormone compositions to induct callus, adventitious buds and roots are respectively:MS+BA 1.0 mg·L -1 +IBA 0.05 mg·L -1 +sugar 3.0%,Ms+BA 2.0 mg·L -1 +IAA 0.5 mg·L -1 +sugar 3.0% or Ms+Kt 3.0 mg·L -1 +IAA 0.05 mg·L -1 +sugar 3.0%,and Ms+NAA 0.3 mg·L -1 +IBA 0.3 mg·L -1 +sugar 3.0%.
