摘要
目的 研究抑癌基因p16 5′CpG岛甲基化状态及其与胃癌发生、发展及浸润转移的关系。 方法 对 46例胃癌组织和 30例良性病变胃粘膜 (对照组 ) ,分别采用甲基化敏感性限制性内切酶 (HpaⅡ、MspⅠ、SacⅡ )酶切后PCR扩增进行p16基因甲基化多位点检测。结果 46例胃癌组织 p16基因甲基化 13例 (2 8.3 % ) ,30例对照组无p16基因甲基化 ,两者比较有显著性差异(P <0 .0 5 )。 2 3例胃癌浸润未达肌层患者 p16基因甲基化 2例 (8.7% ) ,2 3例达肌层或浆膜层患者 p16基因甲基化 11例(47.8% ) ,两者比较有显著性差异 (P <0 .0 5 )。 2 9例高中分化胃癌患者无 p16基因甲基化 ,17例低分化胃癌p16基因甲基化 13例(76 .5 % ) ,两者比较差异有显著性 (P <0 .0 5 )。 18例呈结节生长型胃癌p16基因甲基化 6例 (33 .3 % ) ,2 8例局限溃疡或溃疡浸润型胃癌 p16基因甲基化 7例 (2 5 .0 % ) ,两者比较无显著性差异 (P >0 .0 5 )。 2 0例贲门、胃底、胃体癌 p16基因甲基化 5例 (2 5 .0 % ) ,2 6例胃角、胃窦癌 p16基因甲基化 8例 (30 .8% ) ,两者比较无显著性差异 (P >0 .0 5 )。 9例伴肝或淋巴结转移者胃癌p16基因甲基化 4例 (44 .4% ) ,37例无转移者p16基因甲基化 9例 (2 4.3 % ) ,两者比较无显著性差异 (P >0 .0 5 )。
Objective To investigate the relationship between methylation of the 5′CpG island of the p16 tumor suppressor gene and carcinogenesis,depth of invasion and metastases of gastric cancer.Methods PCR based methylation assay was applied to examine a total of 46 samples of primary gastric cancer and 30 samples of control group for p16 gene methylation in Hpa Ⅱ,Msp Ⅰ,Sac Ⅱ sites.Results The methylation rate of the 5′CpG island of p16 gene was 0% in 30 samples of control group,and it was 28.3% in 46 samples of gastric cancer,there was significant difference beween two groups( P <0.05 ).The methylation rate of p16 gene was 47.8% and 8.7% in gastric cancer invading muscular layer and non invading group, respectively( P <0.05).The positive rate in poorly differentiated gastric cancer was 76.5% and significantely higher than in highly and moderately differentiated gastric cancer(0%)( P <0.05).The methyltion rate of p16 gene was detected in 44.4% of the gastric cancer with liver or lymph nodes metastases and in 24.3% of gastric cancer without metastases,there was no significant difference( P >0.05).The methylation rate was 25.0% in cardia and body of stomach cancer and 30.8% in angle of stomach and antrum( P >0.05).Conclusion The methylation of the 5′CpG island of the p16 tumor suppressor gene plays an importment role in gastric carcinogenesis.It is also suggested that the methylation of p16 gene is related to the depth of invasion and grade of differentiation of gastric cancer.There are no association between the methylation of p16 gene and growth ways,locations and metatastases of gastric cancer.
出处
《实用癌症杂志》
2001年第3期284-287,共4页
The Practical Journal of Cancer
