摘要
目的 观察肾癌细胞凋亡的形态学特征及凋亡过程中线粒体结构的变化 ,揭示线粒体在细胞凋亡过程中的作用。方法 采用 3种化疗药物诱导肾癌细胞株发生凋亡 ,再于光镜和电镜下观察肾癌细胞的形态结构改变。结果 3种药物均可诱导肾癌细胞凋亡 ,有典型的细胞凋亡的形态学改变 ,线粒体结构改变早于细胞染色质结构改变。结论 线粒体在细胞凋亡的过程中起着重要的调控作用 。
Objective To study the morphological character of apoptosis in renal cell cancer and the ultrastructure and function change of mitochondria during the process of apoptosis.Methods Apoptosis in renal cancer cell line was induced by three antineoplastics. The morphological and structural change of renal cancer cells during the process of apoptosis were observed by microscope and electron microscope.Results Apoptosis in renal cancer cell line could be induced by three antineoplastics.The specific morphological change of apoptosis had occurred.The ultrastructural change of mitochondria was earlier than the change of chromatin.Conclusion The mitochondria may take an important role in apoptosis.The component in the mitochondria might promote or impede the process of apoptosis.
出处
《实用癌症杂志》
2001年第3期236-238,共3页
The Practical Journal of Cancer
