摘要
研究了猕猴桃“海沃特”成熟果实各组织中ACC氧化酶基因的转录水平和翻译水平对乙烯生成的影响 .结果表明 :①乙烯生成具有组织差异性 ;②ACC氧化酶基因mRNA的表达在各组织中水平相似 ;③ACC氧化酶的表达水平具有显著的组织差异性及时间顺序性 .因此 ,ACC氧化酶的表达水平的高低可能是导致乙烯生成的组织差异性的主要原因之一 .
The effect of the expression of 1-aminocyclopropane-1-carboxy-lic acid (ACC) oxidase gene on the ethylene production in outer-pericarp, inner-pericarp and columella of ripening Hayward kiwifruit (Actinidia deliciosa) is studied. The results indicate that (1) the ethylene production rates varys in different tissues; (2) The transcript levels of ACC oxidase gene mRNA are similar in all tissues, and (3) ACC oxidase protein is expressed differently in fruit tissues and in sequence. In conclusion, the differences of ethylene production among different tissues may be caused primarily by different expressions of ACC oxidase.
