摘要
用SPF鸡胚从疑为鸽新城疫的云南省某信鸽场分离到一株毒株。该毒株能凝集鸡红细胞 ,且其凝集作用能被抗新城疫阳性血清抑制 ;该毒株对氯仿、酸敏感 ;在病毒中和试验中 ,抗NDV阳性血清对该毒株中和效价为 1∶2 1.75;将病毒分离物回归鸽子 ,复制出与自然病例相似的临床症状 ,且全部感染鸽都发生了血清学抗体转换 ;静脉接种 6周龄雏鸡 ,10天后HI价显著增高 ,且其IVPI为 0。试验结果表明该毒株为鸽新城疫病毒。
One strain virus was isolated from affected pigeons of showing Newcastle disease syndrome by SPF chicken embryo.This strain could agglutinate the erythrocytes of chicken,the hemagglutination of virus could be inhibited by anti NDV serum.The virus was sensitive to chloroform,acid.The titer of neutralization reaction was 1∶1 1.75 .When isolate was inoculated to pigeons,all affected pigeons took place seroconversions and showed clinical symptoms.HI titer of 6_week_old chicken enhanced obviously after intravenous injection 10 days,and their IVPI was 0. It was demonstrated that Yunnan isolate was pigeon Newcastle disease virus.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2001年第3期191-193,共3页
Chinese Journal of Preventive Veterinary Medicine
关键词
鸽
新城疫病毒
分离鉴定
HA价
HI价
Pigeon
Newcastle disease virus
Isolation and identification
HA titer
HI titer
