摘要
目的与方法 :为探Wilms瘤中 p16基因表达与肿瘤病理分型和临床分期之间的关系 ,应用免疫组织化学SP方法 ,对 5 2例Wilms瘤及 7例肿瘤周围正常肾组织 (对照 )手术标本中 p16基因的表达情况进行检测。结果 :5 2例wilms瘤中有 16例 p16基因表达阳性 ,阳性率为 30 .8% ,其中预后良好型 p16基因表达阳性率为 37.2 % (16 /4 3) ,预后差者为 0 % (0 /9) ,均明显低于正常对照 10 0 % (7/7) ,差异具有显著的统计学意义 (P <0 .0 5 )。在Ⅰ、Ⅱ、Ⅲ、Ⅳ临床期中 ,p16表达阳性率以Ⅰ期最高 ,逐步递减 ,分别为 47.6 % (10 /2 1)、37.5 % (3/8)、2 8.6 % (2 /7)、6 .3% (1/16 )。结论 :Wilms瘤中 p16基因表达与肿瘤病理分型和临床分期存在联系 ,p16基因失活似是Wilms瘤发生发展中的晚期事件。
Purpose and Methods: In order to investigate the p16 gene expression and its relationship with pathological type and clinical stage, 52 Wilms tumor specimens and 7 normal renal tissues aroud tumor were stained routinely by SP immunohistochemical method. Results: Positive expression rate of p16 gene was 30.8% (16/52) in tumor specimens and significantly lower than that in control 100% (7/7) (P<0.05). The positive rates were 37.2% (16/43), 0% (0/9) in the favorable pathological type and the unfavorable pathological type, and 47.6% (10/21), 37.5% (3/8), 28.6% (2/7),6.3% (1/16) in Ⅰ,Ⅱ,Ⅲ,Ⅳ clinical stage respectively. Conclusion: The positive expression of p16 gene was correlated with pathological type and clinical stage, and p16 inactivation seems to be the later event of Wilms tumor progression.
出处
《癌变．畸变．突变》
CAS
CSCD
2001年第2期96-98,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
