摘要
目的 :探讨白细胞介素 6 (interleukin 6 ,IL 6 )及可溶性IL 6受体在多发性骨髓瘤 (multiplemyleoma ,MM)病人外周血细胞中的表达及作用。方法 :取多发性骨髓瘤患者 (缓解 2 7例 ,未缓解 19例 )及正常对照者 (2 9例 )血清 ,利用‘7TD1细胞检测患者血清中IL 6的生物学活性 ,双抗体夹心ELISA法测定其中的sIL 6R水平。采用DNA结合蛋白的凝胶阻滞电泳检测MM患者的外周血单个核细胞中急性期蛋白反应因子 (acutephasereactionfactor,APRF)活性。结果 :MM患者血清中IL 6及sIL 6R水平均显著高于正常对照 ,且其水平与骨髓瘤负荷量呈正相关。DNA结合蛋白的凝胶阻滞电泳结果表明 :MM患者外周血单个核细胞中急性期反应因子APRF活性表达增强 ,而健康人外周血单个核细胞中无APRF活性的表达。结论 :在MM条件下 ,IL 6信号转导途径中的某些调控机制 (如蛋白激酶等 )发生改变 ,从而使得核内的APRF激活转录 ,提示IL
SUMMARY Objective: To study the expression and role of IL 6, IL 6 receptor(IL 6R) and acute phase reaction factor (APRF) in PMNC of multiple myeloma(MM) patients. Methods:The bioactivity of IL 6 and the level of sIL 6R in sera of MM patients were measured using IL 6 dependent cell line 7TD1 and ELISA respectively. The APRF activity of PMNC DNA binding protein was observed by gel blocking electrophoresis (EMSA). Results:The bioactivity of IL 6 and the level of sIL 6R in MM sera were significantly higher than those of the control and the level was coordinated with that of the tumor. The results of PMNC DNA binding protein EMSA showed that PMNC of MM patients expressed APRF after stimulation of IL 6,whereas there was no activity of APRF in the normal PMNC. Conclusion:In MM,some regulation machines of IL 6 signal transduction pathways changed (eg.protein kinase), resulting in the activation of transcription of acute phase reaction element(APRE), APRF may have a role in pathogenesis of MM.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2001年第2期170-172,共3页
Journal of Peking University:Health Sciences
关键词
白细胞介素-6
多发笥骨髓瘤
急性期蛋白反应因子
Interleukin 6/blood
Receptors, interleukin/blood
Multiple myeloma/blood
Acute phase reaction factor
