摘要
在对人胚成骨样细胞分离培养的基础上，进一步探讨不同剂量转化生长因子－β（ＴＧＦ－β）对人胚成骨样细胞ＤＮＡ、胶原蛋白和碱性磷酸酶合成的影响。结果表明：ＴＧＦ－β对体外培养的人胚成骨样细胞ＤＮＡ合成有抑制作用，对胶原蛋白和碱性磷酸酶合成有促进作用。这两方面的作用在ＴＧＦ－β０．０１～１ｎｇ／ｍｌ间呈剂量依赖性，１ｎｇ／ｍｌ时的作用达到最强。本研究提示ＴＧＦ－β在骨形成中的主要作用可能在于介导机体系统因素对骨细胞的作用。
Osteoblastlike cells were isolated from embryonic human calvarium with modified enzyme digestion method. TGFβ which is believed having biological effects on bone cells was added into the culture medium with seperate concentrations. The incorporation of 3HTdR, 3HProline of osteoblastlike cells and alkaline phosphatase(ALP) content of the cells were tested to study the effects of the TGFβ on the proliferation of osteoblasts.The results showed that TGFβ increased ALP activity and the synthesis of collagen, and the cell proliferation was inhibited. It affected the proliferation and differentiation of osteoblastlike cells with the best 1 ng/ml among four concertration of 0.01￣10 ng/ml. These findings suggested that function of TGFβ might be modified by other local growth factors.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
1998年第1期65-68,72,共5页
West China Journal of Stomatology
基金
国家教委博士点基金
关键词
TGF-Β
人胚成骨样细胞
增殖
分化
骨生长因子
骨缺损
\ TGFβ\ \ human osteoblastlike cell\ \ proliferation and differentiation\ \ bone growth factor
