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B7-1基因修饰的肿瘤细胞疫苗抗肿瘤的实验研究 被引量:2

The experimental study on antitumor effect of B7-1 gene modified murine EL-4 cells vaccine
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摘要 目的 :研究B7 1基因修饰的肿瘤细胞作为瘤苗的抗肿瘤作用。方法 :通过逆转录病毒载体 ,将小鼠B7 1基因导入EL 4淋巴瘤细胞中 ,研究EL 4/B7 1在同系C5 7BL/ 6小鼠中的成瘤性及其诱导抗肿瘤免疫的效果。结果 :转B7 1基因诱导了CD80的高表达 ,EL 4/B7 1细胞在小鼠中的成瘤性下降。在肿瘤生长的早期对实验性荷瘤小鼠进行治疗 ,基因修饰的瘤苗作用明显增强。EL 4/B7 1细胞能有效地保护野生型肿瘤细胞的攻击 ,射线灭活的EL 4/B7 1瘤苗作用减弱 .结论 :B7 1基因修饰的肿瘤疫苗可诱导体内的抗肿瘤免疫反应 ,导致肿瘤的部分根除 。 Objective:To study the vaccine potency of gene-modified tumor cells. Methods:The EL-4 lymphoma was transduced with recombinant retrovirus containing the murine B7-1 gene. The effect of gene transduction on antitumor immunity was investigated.Results:The appearance, growth rate and surface marker of MHCⅠand MHCⅡ molecules of EL-4 cells transduced with B7-1 gene were the same with control cells except for CD80 positive in B7-1 gene transduced cells. B7-1 gene transduced EL-4 cells resulted in remarkable loss of tumorigenicity in syngenetic mice. EL-4/B7-1 cells could induce system protective immunity. Therapeutic vaccine of EL-4/B7-1 cells could retard the growth of established early-stage EL-4/Wt tumor significantly, but not retard the growth of late-stage EL-4/Wt tumor. Irradiated EL-4/B7-1 vaccine showed weak effect against challenged EL-4 cells.Conclusion:B7-1 gene transduced EL-4 cells can induce system protective immunity. It suggested that this vaccine have a potential application value in human cancer treatment.
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2001年第5期249-251,共3页 Chinese Journal of Immunology
基金 国家"九五"重点攻关项目资助! (96 90 6 0 1 2 0 )
关键词 B7-1 肿瘤疫苗 基因治疗 基因修饰 B7-1 Tumor vaccine Gene therapy
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  • 1Nakazaki Y,Gene Therapy,1998年,5卷,10期,1355页 被引量:1

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