摘要
本实验用 AS 模型家兔β-VLDL 和 VLDL 分别与小鼠腹腔巨噬细胞一起培养,测定细胞内 Ap(?)-B,TC、FC 和 CE 水平变化并观察细胞形态学改变。结果表明:β-VLDL 使巨噬细胞内脂质含量增加并使其转化为泡沫细胞,培养前后两者细胞内 Apo-B,TC,FC 和 CE 含量分别为30.6±3.4,148.6±6.1,49.8±2.8和98.8±10.7μg/mg,细胞蛋白质,差别均有高度显著性(P<0.01);VLDL 组培养前后的差别相应为12.3±2.5,24.0±2.4,11.24±1.6和12.9±2.7μg/mg·细胞蛋白质,差别亦均有高度显著性(P<0.01).两组问差异亦同样均有高度显著性(P<0.01).文中对两种脂蛋白与泡沫细胞的关系进行了讨论。
β-very low density lipoprotein (β-VLDL) isolated from cholesterol-fed rabbit was used to stimu- late cholesterol ester synthesis and accumulated in mouse peritoneal macrophages. Mouse peritoneal macrophages were incubated with β-VLDL and VLDL respectively.Then mea- sured was the contents of Apo-B,TC,FC,and CE in the macrophages.The contents of Apo-B,TC, FC,and CE were increased by 36.0±3.4,148.6±6.1,49.8±2.8 and 98.8±10.7μg/mg in the cell protein respectively in β-VLDL group,(P<0.01);numerouse cytoplasmic lipid drouplets were ob- served under the electron microscope,The contents of Apo-B,TC,FC and CE were increased 12.3± 2.5,24.0±2.4,11.2±1.6 and 12.9±2.7μg/mg·cell.protein respectively in VLDL group.Al though lipids and Apo-B increased significantly but no lipids droplets were seen in the cytoplasma.
关键词
脂蛋白
巨噬细胞
胆固醇
生理学
lipoproteins,VLDL/ME,macrophages/PH
foam cell/PH
cholesterol/ME
