摘要
目的 :以运动、外源性补充CoQ以及运动合并补充CoQ作为干预手段 ,观察线粒体能量转换速率、质子漏与活性氧产生之间的相互关系 ,进一步探讨运动性内源活性氧生成和代谢途径及可能机制。方法 :雄性SD大鼠 36只 ,随机分为 :1)安静对照组 (R ,n =6 ) ;2 )运动中对照组 (Em ,n =6 ) ;3)力竭对照组 (Ei,n =6 ) ;4)安静补药组 (QR ,n =6 ) ;5 )运动中补药组 (Qm ,n =6 ) ;6 )力竭补药组 (Qi,n =6 )。采用三级递增负荷力竭运动模型 ,测定心肌线粒体ROS产生速率、线粒体膜电子传递与质子泵出比值 (H+/ 2e)以及线粒体态 4呼吸速率。结果 :(1)Em和Qm组心肌线粒体ROS产生、H+/ 2e和态 4呼吸分别比R和QR组显著增高 ,并且 ,Qm组三项指标显著高于Em组。 (2 )相关分析表明 ,ROS产生速率分别与H+/ 2e和态 4呼吸速率呈正相关。结论 :运动所致的心肌线粒体质子跨膜势能升高引发了活性氧的生成增加 ,并进而增加质子漏 ,“活性氧循环”与Q循环和质子循环并存和共同运转可能是运动性内源活性氧生成及代谢的重要机制。
Objective In order to further explore the possible mechanism of exercise-induced endogenous ROS including their generation and metabolic pathway, we observed the relationships between myocardial mitochondria energy transforming rate, proton leak and ROS generation with intervention of exercise, CoQ supplement and combination of CoQ supplement and with exercise . Methods SD rats were forced to run on treadmill using Bedford three-step incremental protocol. The animals were divided into six groups: 1. Normal rest control (R); 2. Exercise midway control (Em); 3. Exhaustion control (Ei); 4. CoQ supplement rest (QR); 5. Supplement of CoQ combined with midway exercising (Qm); 6. Supplement of CoQ combined with exercising to exhaustion (Qi). Following parameters in myocardial mitochondria after exercise were determined: 1. The rate of proton pump out and electron transfer of respiratory chain I+III (H +/2e) in the present of malate and pyruvate; 2. ROS generation rate; 3. State 4 respiration rate. Results 1. ROS, H +/2e and state 4 in Em and Qm were significantly higher than those in R and QR, respectively. These parameters in Qm were significantly higher than those in Em. 2. Correlation analysis showed ROS generation was positively related to H +/2e and state 4 respiration. Conclusion High proton transfer electron-chemistry potential energy outside membrane during exercise led to ROS generation, which then increased proton leak. Coexistence of “reactive oxygen cycle' with “Q cycle' and “proton cycle' could be an important mechanism of exercise-induced endogenous ROS generation and metabolism.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2001年第2期134-138,共5页
Chinese Journal of Sports Medicine
基金
国家体育总局局管课题和天津市教委科技发展基金! (No :9916 0 1)资助
