摘要
研究了以血红蛋白（Ｈｅｍｏｇｌｏｂｉｎ，Ｈｂ）作为过氧化物模拟酶对过氧化氢－４－氨基安替比林（４－Ａｍｉｎｏａｎｔｉｐｙｒｉｎｅ，４－ＡＡＰ）氯取代苯酚衍生物显色体系的催化反应性能，探讨了不同氯取代苯酚类衍生物作为酶催化反应氢供体底物的构效关系及酶催化反应的可能机理。拟定了Ｈｂ催化Ｈ２Ｏ２氧化４－ＡＡＰ－２，３，个三氯苯酚（２，３，４－Ｔｒｉｃｈｌｏｒｏｐｈｅｎｏｌ，ＴＣＰ）显色体系用于Ｈ２Ｏ２的测定方法。该方法测定Ｈ２Ｏ２灵敏度高，表观摩尔吸光系数为 ２．２１×１０４ Ｌ·ｍｏｌ－１·ｃｍ－１。将拟定方法与葡萄糖氧化酶催化反应偶联，用于人血清样品中葡萄糖含量的测定，得到满意的结果。
The enzyme catalytic reaction properties of H2O2-4-aminoantipyrine (4-AAP)-the different chlorophenolic derivatives color systems using hemoglobin as enzyme mimetic of peroxidase was studied. Meanwhile, the relationship of structure-effect for the different chlorophenolic derivatives as substrate has been discussed, and the mechanism of catalytic reaction has also been investigated. The method for the determination of hydrogen peroxide was proposed using 4-AAP-2,3,4 trichlorophenol system with hemoglobin as catalyst. The sensitivity of method proposed for the determination of H2O2 was better than other similar systems. The apparent molar absorptivity of the chromogenic reaction for H2O2 was 2.21 x 10(4) L(.)mol(-1) cm(-1). Satisfied results were obtained in the determination of H2O2 in synthetic samples by this method. Also, the method was coupled with glucose oxidation reaction for the determination of glucose in human serum.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2001年第4期378-382,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助项目!(No.29775015)
