摘要
目的 :探讨二磷酸盐类药物——阿可达 (帕米磷酸钠 )对人工关节无菌性松动的影响。方法 :随机选择准备行人工关节置换术患者 17例。采集外周血 ,分离单核细胞 ,每例标本分成 5份 ;A组 :仅单核细胞 ,为对照组 ;B组 :单核细胞 +骨水泥微粒 ;C组 :单核细胞 +骨水泥微粒 +阿可达 (5 μg/ ml) ;D组 :单核细胞 +骨水泥微粒 +阿可达 (10 μg/ ml) ;E组 :单核细胞 +骨水泥微粒+阿可达 (2 0μg/ m l)。培养 48h后 ,放免法测定细胞上清中肿瘤坏死因子 (TNF)含量。结果 :单核细胞 +骨水泥微粒组的 TNF含量明显高于对照组 (P<0 .0 1) ;单核细胞 +骨水泥微粒 +阿可达组的 TNF含量明显低于单核细胞 +骨水泥微粒组 (P<0 .0 1)。而不同浓度阿可达组之间的差异无统计学意义 (P>0 .0 5 )。结论 :骨水泥微粒可刺激单核细胞分泌 TNF,而阿可达能有效地抑制单核细胞的这种分泌。二磷酸盐能防治人工关节置换术后由 TNF等溶骨性因子引起的人工关节周围的骨溶解 。
Objective:To explore the effects of bisphosphonate on aspetic loosening of prothesis. Methods:The human blood was divided into five groups. Group A: only mononuclear cells, which is control group; group B: mononuclear cells+PMMA particles; group C: mononuclear cells+PMMA particles+Pamidronate(5 μg/ml); group D: mononuclear cells+PMMA particles+Pamidronate(10 μg/ml); group E: mononuclear cells+PMMA particles+Pamidronate(20 μg/ml). Each group was cultured for 48 hours, and then the TNF of each group was determined. Results:The levels of TNF were much higher in group B than in group A,C,D and E ( P<0.01 ). But there were no significant difference in group C,D, and E ( P >0.05). Conclusion:It shows the PMMA particles could stimulate mononuclear cells to excrete TNF, which can be inhibited by bisphosphonates. By inhibiting bone resoption associated with the particles and TNF, Pamidronate and other preparation of bisphosphonates may have clinical utility in the prevention and treatment of aspetic loosening of prosthesis.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第3期208-210,共3页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省教委科研基金资助项目 ( 99KJB3 2 0 0 0 1)
