摘要
根据葡萄糖酸亚铁在pH4.25的缓冲溶液中,与邻菲啰啉试剂作用生成橙红色配合物的特性。用分光光度法在波长515nm处,测定该配合物的吸光度。Fe(Ⅱ)浓度范围在0.5～5μg·ml^(-1),符合比耳定律。试验中如有Fe(Ⅲ)存在,加入盐酸羟胺还原,亦可应用本法测定其它制剂中Fe(Ⅱ)含量。本法与美国药典(ⅩⅪ版)硫酸铈法比较,结果一致。
In an acetic acid buffer of pH 4.25, Ferrous Gluconate reacts with 1, 10-phenanthroline to form an orange-red complex. The absorbance was measured at 515nm against a reagent blank. Beer's law is obeyed over the concentration range 0.5～5μg/ml of ferrous. Ferrous Content can be determined with this method in pharmaceuticals also. The results showed good agreement with those obtained by cerium sulphate method.
关键词
葡萄糖酸亚铁
分光光度法
片剂
spectrophotometry
ferrous gluconate
cerium sulphate method
