摘要
目的 检测诊断超声与细胞凋亡相关基因P5 3和Bcl 2mRNA的关系。方法 3 2只SD雄性大鼠随机分为 4组 :对照组、10min组、2 0min组和 3 0min组 ,应用HP 85 0 0彩色血流显像仪 ,探头频率7.5MHz ,超声输出功率 6.8mW ,空间平均时间平均声强 3 .4mW /cm2 ,对上述各组大鼠睾丸组织直接辐照 ,辐照后 2 4h取材 ,应用原位杂交技术检测P5 3及Bcl 2mRNA表达改变。结果 诊断超声辐照≤ 10min不会引起大鼠睾丸组织P5 3及Bcl 2mRNA的表达改变 ,2 0min组P5 3mRNA表达率明显增加 ,达( 5 2 .63± 5 .89) % ,3 0min组继续增加达 ( 60 .47± 6.5 7) % ;而Bcl 2mRNA在 2 0min组则明显下降为( 3 4 .96± 6.48) % ,3 0min组继续下降为 ( 3 1.0 1± 5 .67) %。结论 诊断超声辐照大鼠睾丸组织 >10min可引起凋亡诱导因子P5 3mRNA高表达 ,凋亡抑制因子Bcl 2mRNA低表达。
Objective To investigate the changes in the expressions of P53 mRNA and Bcl 2 mRNA in spermatogenic cells in rats after exposure to ultrasound to elucidate the possible mechanisms underlying the apoptosis induced by ultrasound at genetic trascription level. Methods Thirty two healthy male SD rats,(30±2) days old and (70±5) grams body weight, were randomized into 4 groups (n=8) according to the time of exposure to ultrasound,including control group without exposure,groups of 10 minutes, 20 minutes and 30 minutes with exposure to ultrasound for 10,20 and 30 min,respectively. Samples were taken 24 hours after exposure to ultrasound,then 4% paraformal dehyde fixed,paraffin embedded and sectioned into 4μm sections (slides were pretreated with APES and poly lysine). P53 and Bcl 2 in situ hybridization kits were purchased from Wuhan Boshida Bio engineering Co.Results There was no significant difference in the expressions of P53 mRNA or Bcl 2 mRNA in sperematogenic cells in rats between the control group and 10 minutes group. The expressions of P53 mRNA in groups over 10 minutes were up regulated and that of Bcl 2 mRNA were down regulated. P53 mRNA in spermatogenic cells in rats was increased from ( 44.13 ± 5.05 )% in control group to ( 60.47 ± 6.57 )% in group 30 minutes. Bcl 2 mRNA was decreased from ( 40.29 ± 9.10 )% in control group to ( 31.01 ± 5.67 )% in group 30 minutes. Conclusions Exposure to ultrasound over 10 minutes can induce up regulatory expressions of P53 mRNA and down regulatory expressions of Bcl 2 mRNA in spermatogenic cells in rats.
出处
《中华超声影像学杂志》
CSCD
2001年第3期182-184,共3页
Chinese Journal of Ultrasonography
