摘要
目的 探讨治疗节段性骨缺损新方法—新型重组合异种骨 (NRBX)与带血循骨膜联合移植方法的效果。方法 将基因重组人BMP2 (rhBMP2 )与去抗原牛松质骨载体 (BCB)复合 ,制成NRBX ;并将之与带血循环骨膜联合移植修复兔桡骨干1 5cm缺损 ,通过X线、生物力学、骨密度、组织学等检测手段 ,对该联合方法治疗节段性骨缺损的效果进行研究 ,并与单纯NRBX移植方法对比。结果 ①单纯NRBX移植 ,可在 16周使节段性骨缺损基本修复 ,其机制与过程与重组合异种骨(RBX)相似 ;②NRBX与带血循骨膜联合移植 ,仅用 8周即可修复骨缺损 ,其修复机制与骨折修复相仿 ,包括膜内成骨和软骨成骨两种机制。结论 上述二种方法均可有效地修复节段性骨缺损 ,但以NRBX与带血循骨膜联合移植较为理想 ,该方法同时具有良好的骨生成。
Objective To study the efficacy of New Reconstituted Bone Xenograft (NRBX) used alone and combined use of NRBX and vascularized periosteal graft in repairing segmental bony defects Methods A new grafting material (NRBX) was made by combining recombinant human BMP2( rhBMP2) and an antigen free bovine cancellous bone (BCB) as a carrier NRBX was used alone, in conjunction with vascularized peristeal graft to repair a 1 5 cm defect in the radius of the rabbit The defect repairing capability for each of the treatment modalities was assessed radiographically, biomechanically, by densitometry and histological studies Results ①NRBX used alone was capable of healing the defect in large part by 16 weeks, with a similar repair process and mechanism to that seen with RBX; ② use of NRBX in conjunction with vascularized periosteal graft exhibited stronger defect repairing power, basically healing the defect by 8 weeks The repair process consisted of intramembranous and endochondral ossification that resembled the physiological repair during the fracture healing; Conclusion Both methods are effective in repairing segmental bony defects, with NRBX used in conjunction with vascularized periosteal graft being preferred, considering the satisfactory osteogenesis, osteoconduction and osteoinduction seen with this method
出处
《骨与关节损伤杂志》
2001年第2期118-121,共4页
The Journal of Bone and Joint Injury
关键词
骨形态发生蛋白
骨移植
异种移植术
骨膜
骨缺损
Bone morphogenetic protein (BMP)
Bone transplantation
Heterotransplantation
Periosteum
