摘要
研究促卵泡激素 (FSH)、人绒毛膜促性腺激素 (hCG)对昆明白小鼠卵母细胞成熟和卵丘扩展的影响 ,以及体外培养时卵丘扩展与卵母细胞成熟之间的关系。FSH可以明显促进次黄嘌呤 (HX)抑制条件下的卵丘 -卵母细胞复合体CEO卵母细胞成熟及卵丘扩展 ,其最佳作用剂量为 10 0IU/L ,且FSH作用 30分钟即可以使CEO获得恢复减数分裂的信息。在HX存在的条件下 ,FSH处理后 10hr,CEO卵丘明显扩展 ,而生发泡破裂(GVBD)则在 16～ 2 0hr明显增加 ,所有卵丘未扩展的CEO中卵母细胞均未发生GVBD。低剂量hCG单独或与FSH共同存在 ,对CEO卵母细胞成熟及卵丘扩展均无明显影响 ;高剂量hCG可以部分抑制FSH对卵母细胞成熟的促进作用。结果表明 :FSH明显促进CEO卵母细胞成熟及卵丘扩展 ,而hCG却不具有此作用。体外培养条件下 (含次黄嘌呤 ) ,卵丘扩展是卵母细胞成熟的前提条件 ,但卵母细胞成熟并不需要卵丘完全扩展。
The present experiments were conducted to evaluate the effect of gonadotropins on Kunming white mouse oocyte maturation and the relationship between cumulus cell expansion and oocyte maturation in vitro . The cumulus cell enclosed oocytes (CEO) were collected from gonadotropins stimulated Kunming white mouse ovaries and were cultured for 24 hr in 4 mmol/L hypoxthantine (HX) supplemented medium (HX medium) and treated with different agents in the following designs: (1) CEO were treated with FSH (10～500 IU/L) to find the optimal dosage of FSH on the oocyte maturation; (2) CEO were treated with 100 IU/L FSH for the different time (0, 10, 30, 60 and 120 min) and then transferred to a new HX medium for the further culture to figure out the time effect of FSH on oocyte maturation and cumulus cell expansion; (3) CEO were treated with different dose of hCG (0～400 IU/L) or hCG plus FSH to evaluate the effect of hCG on oocyte maturation and cumulus cell expansion. The results revealed that FSH stimulated oocyte resumption of meiosis and cumulus expansion of cumulus cell enclosed oocyte(CEO)in the presence of 4 mol/L hypoxathine(HX), with maximal effect occurring at a dosage of 100 IU/L. The oocyte meiotic resumption could be induced by 30 min FSH presentation. By 10 hr after FSH treatment, cumulus cells had already apparently expanded, whereas the significant increase of GVBD did not appear until 16～20 hr in culture. Nearly all the oocytes with cumulus cell expansion underwent the meiotic resumption. But those CEO whose cumulus cell did not expanded didn't undergo GVBD. Low doses of hCG failed to affect the oocyte maturation and cumulus expansion of CEO, however, high doses of hCG could partially inhibit the oocyte maturation stimulated by FSH. Our results indicate that, in the presence of HX, FSH stimulated significantly cumulus expansion and oocyte maturation of CEO. hCG had no stimulation effect on the oocyte meiotic resumption. The cumulus cell expansion occurred 6 to 8 hr before the oocyte resumption o
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2001年第2期203-208,共6页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金资助项目!(No.3 9770 3 96)
国家教委回国人员启动基金
关键词
昆明白小鼠
卵丘扩展
卵母细胞成熟
促性腺激素
Kunming white mouse, Oocyte resumption of meiosis, Cumulus expansion, Gonadotropins
