摘要
The effects of pulsing electromagnetic fields(PEMFs)on cells are very important subjects in the field of bioelectromagnetics.In this experiment,the cytogenetic effects of PEMF on domestic pig lymphocytes were tested in vitro.Pig lymphocytes in RPMI 1640 medium were exposed to PEMFs of 100 kHz and 200 kHz for 12,24 and 48 hours.Chromosomal aberrations(aneuploidy,breaks,gaps,et al)were significantly increased in exposed cultures,and of these aberrations,56%chromosomal or chromatid breaks and 42%gaps induced by PEMFs were the points of pig chromosomal fragile sites.The baseline frequency of sister chromatid exchange(SCE)increased after exposing lymphocytes continuously to PEMFs of 100 kHz and 200 kHz for 48 hours.These results suggested that the exposure to PEMFs might induce a type of DNA lesion and chromosomal aberrations.
以家猪外周血淋巴细胞为材料,研究了脉冲电磁场(pulsingelectromagneticfields,简称PEMFs)对细胞的遗传学效应。实验发现,100和200kHz的PEMFs对家猪的淋巴细胞照射培养12、24、48h后,染色体畸变(包括非整倍体、染色体断裂等)频率明显高于对照组(P<005)。其中,56%的染色体或染色单体断裂和42%的间隙发生在家猪常见染色体脆性位点部位。同时,经100kHz和200kHz的PEMFs照射48h后,淋巴细胞姐妹染色单体交换(SCE)频率也明显高于对照组(P<005)。实验结果表明,PEMFs能诱导DNA损伤和染色体畸变。
基金
国家自然科学基金资助项目
