摘要
目的 研究细胞毒性T淋巴细胞相关抗原 (CTLA4Ig)对新鲜同种异体骨移植后血淋巴细胞亚群的影响。方法 在组织相容性抗原完全不同的两组小鼠间进行异位骨移植 ,于移植后第 0、1天 ,腹腔注射CTLA4Ig 10 0 μg ,分别于术后 2、4、6周取受体外周血 ,利用流式细胞仪技术测定血淋巴细胞亚群CD4 和CD8。结果 术后第 2周 ,骨移植对照组CD4 亚群 (64 .2 6± 5 .69)和CD4 /CD8比值 (4.98± 0 .5 9)较同系对照组 (49.5 0± 2 .3 2 ,6.96± 0 .5 7)明显升高 (P <0 .0 1,P <0 .0 5 ) ,术后第 4周 ,骨移植对照组CD4 亚群 (62 .48± 3 .78)较同系对照组 (5 4.5 1± 3 .44)仍明显升高 (P <0 .0 5 ) ,术后第 6周时 ,各组间淋巴细胞亚群差异已无显著性 (P >0 .0 5 ) ;同种异体骨移植后注射CT LA4Ig组 (第 2周 5 3 .3 7± 4.17,第 4周 5 5 .3 7± 3 .0 6)与注射对照剂组 (第 2周 64 .2 6± 5 .69,第 4周62 .48± 3 .78)比较 ,CTLA4Ig明显抑制了CD4 亚群的增殖 (P <0 .0 5 ) ,与同系骨移植组相似。结论 利用CTLA4Ig阻断第二信号 。
Objective To investigate the effect of cytotoxic T lymphocyte associated antigen 4 Ig (CTLA4Ig) on blood T lymphocyte subsets in transplantation of fresh allogeneic bone. Methods The bone of C57BL/6 mice was heterotopicly implanted in the BALB/C mice's muscle pouch. Then, CTLA4Ig was administrated by intraperitoneal injection at day 0,1, and the blood specimens of recipients were harvested at 2, 4 and 6 weeks after implantation. A series of T lymphocyte subsets of CD 4, CD 8 and CD 4/CD 8 were measured by using flow cytometry method. Results The CD 4 T lymphocyte subsets and CD 4/CD 8 ratio were significantly higher in the fresh allogeneic bone grafts (control) than those in syngeneic grafts at second week after implantation (64.26±5.69 vs 49.50±2.43, P <0.01; 4.98±0.59 vs 3.90±0.57, P <0.05, respectively). CD 4 subsets were also higher at 4th week (62.48±3.78 vs 54.51±3.44, P <0.05), but no difference in CD 8 subsets. There was no difference among different groups at 6th week( P >0.05). CTLA4Ig (100 μg for 2 days) significantly inhibited the proliferation of CD4 T lymphocyte subsets as compared with the control group at second and 4th week (53.37±4.17 vs 64.26±5.69 at second week; 55.37±3.06 vs 62.48±3.78 at 4th week, P <0.05), which was similar to the syngeneic group. Conclusion CTLA4Ig at a dose of 100 μg for 2 days significantly inhibited cellular immunity of T lymphocyte subsets aroused by the fresh allogeneic bone grafts, and had markedly advantage compared with the immunosuppressants.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2001年第2期129-130,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目 (39670 72 5)
