摘要
本文通过制备荧光素标记庆大霉素和抗庆大霉素兔血清，建立了人血清中庆大霉素的荧光偏振免疫分析技术。方法的灵敏度0．3mg/L,批内、批间误差CV均小于6％，平均回收率104％，合用其它抗生素不干扰测定结果，与微生物法及放射免疫法比较相关系数γ分别为0．9687和0．9544。荧光偏振免疫法的建立，为临床测定床大霉素浓度提供了一种快速、精确和灵敏的新方法。
A fluorescence polarization immunoassay(FPIA) for gentamicin in human serum was es-tablished by preparing the fluorescent-labled ge-ntamicin and antisera specific for the compou-nd. The low limit of the method was 0.3mg/L. CVs within and between-run were<6%. An-alytical recoveries of drugs from serum aver-aged 104%. Commonly, co-administered drugs didnot interference the assay. Comparison of FPIAwith microbiological assay and radioimmunoass-ay for gentamicin yielded correlations of 0.97and 0.95 respectively. The FPIA was shown t?be a precise and reliable procedure for determi-ning gentamicin in human serum.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1990年第1期25-27,共3页
Chinese Pharmaceutical Journal
关键词
荧光偏振免疫分析法
庆大霉素
治疗药物监测
fluorescence polarization immunoassay
gentamicin
therapeutic drug monitoring
