摘要
用酶的抑制剂使精子顶体蛋白酶失活,为目前避孕方法中的一种途径。本文报道用明胶薄层作为蛋白质底物进行酶活性测定的结果,表明慈菇蛋白酶抑制剂及天花粉蛋白酶抑制剂对人、免、大鼠、仓鼠及小鼠精子顶体蛋白酶有明显的抑制作用,并随着剂量增加抑制效应呈增强的趋势。用笨甲酰精氨酸乙酯(BAEE)作底物同样证明慈菇蛋白酶抑制剂对人精子顶体蛋白酶有强的抑制效应。
The inactivation of sperm acrosin by proteinase inhibitors is a way for contraception at themoment.Acrosomal proteinase activity as determined with a thin layer gelatin as a protein substrateindicated that trichosanthes proteinase inhibitor and arrowhead proteinase inhibitor exerted significanteffects on sperm acrosins of human.rabbit,rat,hamster and mouse and their inhibitory effects wereenhanced by increasing the amount of the inhibitor.The results obtained from BAEE substrate alsoshowed that the inhibitory effect of the arrowhead proteinase inhibitor on human sperm acrosin wassignificant.
出处
《生殖与避孕》
CAS
CSCD
北大核心
1993年第2期114-119,共6页
Reproduction and Contraception
关键词
蛋白酶
抑制剂
精子
顶体蛋白酶
Thin layer of gelatin,Arrowhead proteinase inhibitor,Trichosanthes proteinase inhibitor,Sperm acrosin
