摘要
合成了一种具有交联结构的多孔性甲状腺素(T_4)固相放射免疫测定载体微球,并对载体微球的功能基转化、悬臂的引入和功能基的活化反应进行了研究。用合成的载体微球与甲状腺素抗体偶联,通过测定甲状腺素的实验证明,该微球具有较高的结合率。
Porous microspheres with diameter ranging from 250 to 400 mesh and pore sizes from 1524 to 4 304 A were synthesized by suspension polymerization of MMA and DVB with 26-50% paraffin liquid or 30-50% 200# petrol by weight as porogenic agents. The microspheres extracted with petroleum ether were hydrolyzed (50% NaOH, 140-150℃; 1-5 h). The microspheres with spacer were prepared by reacting carboxylated microspheres with 1,6-hexamethylene diamine. The T4 -anti-body was covalently coupled to the microspheres with spacer by a two-step glutaraldehyde method. The reactivity of the microsphere-anti-body conjugates was characterized by reacting the microsphere-anti-body conjugates with 125I labeled thyroxine, the binding percentage is 94%.The results of our experiments showed that the porous microspheres is a ideal carrier for solid phase radioimmunoassay of thyroxine.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1989年第6期638-641,共4页
Chemical Journal of Chinese Universities
