摘要
本文报道了用细胞松驰素B、高温和低温休克等方法诱导华贵栉孔扇贝（Chloamysnobilis）三倍体的实验结果。在26.0～28．0℃条件下，选用不同浓度的细胞松驰素B0．1、0．3、0．5、0．7和1．0mg／dm3，在受精后15min，处理受精卵15min，三倍体诱导率分别为12．5％、37．5％、68．0％、66．6％和81．0％，其中0．5～1．0mg／dm3浓度的细胞松驰素B对改变倍性最有效。高温休克选用31．0、33.0、35.0和37．0℃处理受精卵，最高三倍体诱导率达46.7％（33．0℃，处理15min）。低温休克选用6．0、8．0、10．0和12．0℃处理受精卵，最高三倍作诱导率为40．7％（10．0℃，处理20min）。实验结果表明，随着处理强度的增大和处理时间的延长，三倍体诱导率增高、但孵化率下降，畸型率上升。用高温休克处理的实验组幼虫生长速度较对照组快。作者认为，在商业性生产中，温度休克是诱导扇贝三倍体的一种简便、有效的方法。文中还就细胞松驰素B与温度休克诱导三倍体的机制进行了讨论。
This paper reports triploidy of scallop,Chlamys nobilis induced by cytochalasin B(C. B) and temperature shock. Triploidy induction trials were carried out by C. B treatment for 15min after insemination at five concentrations 0.1, 0. 3, 0. 5, 0. 7, and 1.0mg/dm3, the fre quency of triploid was 12. 5%, 37. 5%, 68.0%, 66. 6% and 81.0%, respectively. The C. B concentration between 0. 5 to 1. 0mg/dm3 was the most effective to induced triploidy. Heat shock was applied to the fertilized eggs at four temperatures 31.0, 33. 0, 35. 0,and 37. 0℃,the highest frequency of triploid was 46. 7% (33. 0℃, 15min). Cold shocked fertilized eggs at four temperature 6. 0, 8.0, 10.0 and 12.0℃,the highest frequency of triploid was 40. 7% (10.0℃, 20min). This trial shows that hatchability decreased obviously and abnormality in creased rapidly with the increasing induction rate of triploidy when treating intensity was in creased and duration prolonged. Daily growth of larvae with heat shock treatment (33.0℃) was higher than the control. The authors consider that temperature shock treatment to be an effective and simple method to induce triploidy from a commercial point of view. In addition,the mechanisms of polyploid inducement with C. B treatment and temperature shock are dis cussed.
出处
《台湾海峡》
CAS
CSCD
1995年第2期155-162,共8页
Journal of Oceanography In Taiwan Strait
基金
福建省自然科学基金
