摘要
采用电镜细胞化学和酶活性的图象定量分析方法，对高压下氧中毒小鼠大脑皮质毛细血管内皮细胞的碱性磷酸酶（ＡＬＰ）活性定位和强度变化进行了研究。许多定量参数表明，高压下发生氧中毒后，小鼠大脑皮质毛细血管内皮细胞ＡＬＰ活性增强。作者讨论了毛细血管ＡＬＰ活性增高与功能改变之间的可能关系。
he Changes of alkaline
phosphatase(ALP) activity in the mice cerebral cortex capillary endotheli-um after hyperbaric
oxygen poisoning(HOP)were studied using the methods of ultra-cytochemistry and image
quantitative analysis,15 ICR mice weighing approxiniately 20～22 g were randomly divided
into 3 groups:the normal (NP) group, the hyperbaric air(HA)group,and the hyperbaric oxygen
poisoning group(HOP).The animals of HA and HOP groups were put into the same hyperbaric
aninial chamber and were given air and 100%oxygen respectively.The pressure was increased
from 0.1 MPa(1 ATA)to 0.5 MPa(5 ATA)in 4 min and the animals were kept at 0.5MPa for 37 min.
The time of decom-pression was 15 min. The cerbral cortex of all the mice were then removed
and fixed according to the method of ultra-cytochemistry. Quantitative parameters of ALP
activity in the cerebral cortex capillary endothelium were measured and calculated by means of
a computer image analyser.The results showed that there were no significant change of
structure and ALP activity of capillary endothelium in the HA group; whereas in the HOP
group,some mitochondria in some neurons and cap-illary endothelium showed vacuolation with
broken cristae and the capillary endothelial membrane was injured;at the same time,the ALP
activity in the membrane increased significantly. The ALP is an unspecialized enzyme which
hydrolyses the phosphate. It is mainly located at the cell membrane and takes part in the
transport process of cells. The increase of ALP activity in the HOP group might mean a
compensative or pathological increase of transporting function. Under this circum-stance,the
blood-brain barrier in the HOP group could be jeopardized
基金
中国人民解放军总后勤部"八.五"科研基金
关键词
碱性磷酸酶
图象分析
高压氧
氧中毒
大脑皮质
Alkaline phosphatase
Image analysis Hyperbaric oxygen Oxygen poisoning cerebral cortex
