摘要
采用ELISA法检测0-14岁人群柯萨奇病毒A组16型（CoxA16）及肠道病毒71型（EV71）抗体水平。在入选的1000例儿童中,EV71 IgM总阳性率19．9%,EV71 IgG总阳性率47.7%,CoxA16 IgM总阳性率22．8%,CoxA16 IgG总阳性率51．3%；EV71 IgM总阳性率与CoxA16 IgM总阳性率比较,差异无统计学意义（χ^2=2．794,P〉0．05）；EV71 IgG总阳性率与CoxA16 IgG总阳性率比较,差异无统计学意义（χ^2=2．793,P〉0．05）。各年龄组比较,新生儿组IgG抗体阳性率较高,其EV71 IgG抗体阳性率41．5%,CoxA16 IgG抗体阳性率49．5%；婴儿组IgG抗体阳性率最低,EV71 IgG、CoxA16 IgG抗体阳性率分别为38．0%,43．5%；除新生儿组外,随年龄增加,人群EV71、CoxA16 IgG逐渐增高,组间差异有统计学意义（χ^2=27．04、19．98, P 〈0．01）；在 IgM 方面,人群EV71、CoxA16 IgM 随年龄逐渐升高,组间差异有统计学意义（χ^2=41．12、37．13,P〈0．01）。两种HFMD病原体的IgM、IgG抗体性别间差异无统计学意义（χ^2=0．51、1．77、0．36、2．12,P〉0．05）。
Enzyme-linked immunosorbent assay （ ELISA） was applied to detect the antibody against CoxA16 and EV71 among 0-14 age groups. In 1 000 cases of children, the total positive rate of anti-EV71 IgM was 19. 9%, the total positive rate of anti-EV71 IgG was 47. 7%, the total positive rate of anti-CoxA16 IgM was 22. 8%, the total positive rate of anti-CoxA16 IgG was 51. 3%. There was a negative correlation between positivity for EV71 IgM and positivity for CoxA16 IgM（χ^2 =2. 794,P〉0. 05）. There was also a negative correlation between positivity for EV71 IgG and positivity for CoxA16 IgG（χ^2 =2. 793,P〉0. 05）. The positive rate of IgG antibody against CoxA16 and EV71 was higher in neonatal group in which anti-EV71 IgG was 41 . 5% and anti-CoxA16 IgG was 49 . 5%. The lowest positive rate of IgG antibody against CoxA16 and EV71 was in infant group in which anti-EV71 IgG was 38. 0% and anti-CoxA16 IgG was 43. 5%. Except for neonatal group,the positive rate of IgG antibody against Cox-A16 and EV71 increased gradually with the age growth, there were statistical differences among the age groups （χ^2=27. 04 and 19. 98,P〈0. 01）. With the age growth,the positive rate of IgM antibody against EV71 and CoxA16 increased,there were statistical differences among the age groups（χ^2 =41. 12 and 37. 13,P〈0. 01）. There were not statistical differences between gender groups （χ^2 =0. 51,1. 77,0. 36 and 2. 12,P〉0. 05）.
出处
《安徽医科大学学报》
CAS
北大核心
2014年第7期1013-1015,共3页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金(编号:090413268X)
