摘要
以花生(Arachis hypogage L.)品种花育22号成熟种子胚小叶为诱变材料,培养在添加平阳霉素的体胚诱导培养基中进行诱变处理,将成活的形成体胚的外植体转移到添加NaCl的体胚萌发培养基中进行耐盐定向筛选。对获得的再生植株M2的部分农艺性状进行观察测定,结果显示,花生胚小叶经平阳霉素离体诱变和NaCl定向筛选获得的再生植株后代表现型存在广泛变异,如主茎高、侧枝长、分枝数、单株结果数、荚果形状和大小、种皮颜色等均发生明显的变异。从后代变异规律可以看出,利用高效低毒的平阳霉素作为诱变剂与组织培养结合可作为创造花生新种质的一种有效手段。
The embryonic leaflets from mature peanut seed （cv. Huayu 22） were cultured on somatic embryogenesisinduction medium containing pingyangmycin （PYM） , the surviving explants with somatic embryos were transferred to a germination medium containing NaCl for direct screening. The plantlets from germinated somatic embryos were grafted and then transplanted in the field. In next year, all seeds from each plant were sown in the field. The mutagenic effects of in vitro mutagenesis with PYM on peanut were studied by analyzing the M2 agronomic traits. The results showed that the variability was extensive on main stem height, length of lateral branch, number of lateral branch per plant, pod shape, pod size and pod number per plant, etc. The variability in M2 offspring demonstrated that in vitro mutagenesis with PYM was effective in peanut germplasm enhancement.
出处
《核农学报》
CAS
CSCD
北大核心
2014年第6期955-960,共6页
Journal of Nuclear Agricultural Sciences
基金
国家"863计划"(2012AA101202)
国家自然基金青年基金(31101178)
山东省自然基金(ZR2012CM014)
山东省农业良种工程
关键词
花生
平阳霉素
离体诱变
农艺性状
Peanut （Arachis hypogaea L. ）
Pingyangmycin
In vitro mutagenesis
Agronomic traits
