摘要
目的:研究急性髓系白血病(acute myeloid leukemia,AML)患者钙黏蛋白相关蛋白(α-catenin,CTNNA1)基因的表达情况并探讨其临床意义。方法:应用实时定量PCR(qRT-PCR)法检测25例非恶性血液病患者(对照组)和92例初诊AML患者(观察组)中CTNNA1基因的表达水平,并分析其与临床参数的相关性。结果:观察组中51例(51/92,55.43%)存在CTNNA1低表达,而对照组未发现CTNNA1低表达(0.00%),两组比较,差异有统计学意义(χ2=17.152,P<0.001);CTNNA1表达水平与AML患者的年龄、性别、外周血白细胞计数、血小板计数、血红蛋白含量、FAB分型、核型分组之间均无相关性(P均>0.05)。ROC曲线分析表明CTNNA1表达水平能有效鉴别观察组和对照组,AUC=0.806。观察组中CTNNA1低表达的患者化疗后完全缓解率高于对照组,但差异无统计学意义(χ2=2.476,P=0.142)。4例初诊AML患者在获得完全缓解后CTNNA1表达水平均较治疗前有所升高。结论:CTNNA1基因低表达可能是AML中的一个常见分子事件,检测其表达可用于AML患者的辅助诊断和疾病状态监测。
Objective： To investigate the expression of the human catenin alpha 1 （ CTNNA1 ） gene in acute myeloid leukemia （AML） and its clinical significances. Methods： The expression of CTNNA1 transcript in bone marrow mononuclear cells from 25 controls （control group）and 92 newly diagnosed AML patients（observation group） were detected by quantitative real-time polymerase chain reaction（qRT-PCR） ; and the relationship between CTNNA1 expression and clinical parameters were analyzed. Results： Down- regulated CTNNA1 expression was found in 51 AML cases（55.43% ） , significantly lower than that in all 25 controls （P 〈0. 001 ）. There was no correlation between The CTNNA1 expression level and the sex, age, blood parameters and FAB subtypes（ both P 〉 0.05 ）. ROC curve analysis revealed that the CTNNA1 expression level could discriminate AML patients from controls effectively （AUC = 0. 806）. There was no statistical difference in the complete remission rate between control group and observation group, though the patients with low CTNNA1 expression in observation group had higher complete remission rate after chemotherapy （x^2 = 2.476,P = 0. 142）. The CTNNA1 expression levels of four patients in the observation group all increased after complete remission. Conclusion： The decreased-expression of CTNNA1 gene may be a common molecular event in AML; and detecting its expression could be used for auxiliary diagnosis and monitoring disease.
出处
《江苏大学学报（医学版）》
CAS
2014年第1期52-55,58,共5页
Journal of Jiangsu University：Medicine Edition
基金
国家自然科学基金资助项目(81270630
81172592)
江苏省科技厅临床医学科技专项课题(BL2012056)
江苏省"333工程"科研项目资助计划(BRA2011085)
江苏省普通高校研究生科研创新计划项目(CXLX12_0673)
镇江市科技基础设施计划项目(SS2012003)
镇江市医学重点人才
镇江市社会发展项目(SH2011056)
