摘要
目的探讨进展期白癜风患者外周血中是否存在辅助性T细胞17(Th17)和调节性T细胞(Treg)免疫失衡。方法收集50例进展期白癜风患者和20例正常人外周血,分为患者组和对照组。流式细胞仪检测2组外周血Th17,Treg细胞比例。结果进展期白癜风患者组CD4+CD25+T细胞比例和CD4+CD25+Foxp3+细胞比例分别为(4.05±0.94)%和(0.05±0.02)%较对照组的(5.17±1.51)%和(0.11±0.06)%显著下降,差异有统计学意义(P均<0.05)。进展期白癜风患者组CD4+IL-17A+T细胞比例为(1.30±0.76)%,正常对照组为(0.74±0.58)%,患者组显著高于正常对照组,差异有统计学意义(P<0.05)。将白癜风患者外周血中CD4+CD25+Foxp3+Treg细胞比例(Treg)与CD4+IL-17A+Th17细胞(Th17)比例进行相关性分析发现,两种细胞之间存在显著负相关关系(P<0.05)。结论Th17/Treg免疫失衡可能参与进展期白癜风的发病。
Objective To investigate if the immune imbalance exists between helper T 17 （Thl7） ceils and CD3 ＋ CD25＋ Foxp3 ＋ regulatory T（Treg）cells in the peripheral blood of patients with progressive vitiligo. Methods Pe- ripheral blood was collected from 50 patients with progressive vitiligo and 20 normal human controls. Flow cy- tometry was performed to determine the percentage of Thl7 and Treg cells in peripheral blood. Results There was a significant decrease in the percentage of Treg cells [ （4.05 ±0. 94）% and（0. 05 ±0. 02）% vs（5. 17 ±1. 51 ） % and （0. 11 ± 0. 06） % ] but an increase in the percentage of Th 17 cells [ （ 1.30 ± 0. 76 ） % vs （0. 74 ± 0. 58 ） % l in patients with progressive vitiligo compared with the normal controls. The percentage of Treg cells in patients was negatively correlated with the percentage of Th17 cells （P 〈 0. 05）. Conclusion The immune imbalance between Th17 and Treg cells may play an important role in the pathogenesis of progressive vitiligo.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2014年第7期684-686,695,共4页
The Chinese Journal of Dermatovenereology
基金
陕西省自然科学基金资助课题(2010JM4020)
