摘要
分析了青天葵及其混伪品matK序列,以期在分子水平建立青天葵及其常见混伪品的鉴别方法。采用一对通用引物对matK基因进行PCR扩增并测序,所得序列用DNAMAN、MEGA等软件进行分析。获得青天葵及其混伪品的matK基因序列长度为587 bp,平均GC含量为32.8%。青天葵的种内遗传距离为0,与混伪品种间遗传距离范围为0.016～0.375。基于matK基因序列构建的NJ聚类树能明显地区别青天葵及其混伪品。因此,应用matK基因序列可以有效鉴别青天葵及其混伪品。
In order to establish the novel method on distinguishing Nervilia fordii and its common adulterants,matK genes from N.fordii and its adulterants were amplified and sequenced using a pair of universal primes.The results revealed that matK genes acquired from N.fordii and its adulterants contained 587 base pairs,and average GC content was 32.8%.The intraspecific distances among N.fordii were 0,whereas the interspecific distance between N.fordii and its adulterants ranged from 0.016 to 0.375.The clustering tree according to Neighbor Joining method and Kimura 2-Parameter model discriminated N.fordii and its adulterants obviously.matK gene is suggested to be an effective biomarker to identify N.fordii and its common adulterants.
出处
《广西植物》
CAS
CSCD
北大核心
2014年第3期299-303,共5页
Guihaia
基金
教育部博士点基金(200805720004)
教育部留学回国人员科研项目(教外司留[2009]1001)
