摘要
顶复门原虫是一类专性的细胞内寄生原虫,包括刚地弓形虫、隐孢子虫、疟原虫、巴贝斯虫和球虫等,是人和动物的重要病原。这类原虫具有相似的亚细胞结构并能分泌与入侵相关的保守蛋白,尤其是在入侵宿主细胞阶段分泌的棒状体蛋白(rhoptry proteins,ROPs)被认为是保护寄生虫入侵和繁殖的关键分子。其中ROP18是具有丝氨酸-苏氨酸激酶活性的ROP2家族蛋白成员之一,在刚地弓形虫入侵宿主细胞阶段发挥重要作用,是纳虫空泡(PV)形成时宿主细胞免疫的抑制因子。随着基因组学和蛋白质组学技术的不断发展,其相关研究也越来越深入。本文以目前研究最多的刚地弓形虫ROP18为主,对其发现历史、结构、分泌及定位、对宿主的毒力机制及应用现状做一综述。
Apicomplexan parasites, such as Toxoplasma gondii, Cryptosporidium spp. , Plasmodium spp. , Babesia spp. , Eimeria spp. etc. , are obligate intracellular protozoa and important pathogens of humans and animals. They have conserved subcellular structures and invasion mechanism. Rhoptry proteins （ROPs）, which released into the host cell, are considered to be the key molecules of invasion and replication of parasites in host cell. ROP18, one of active serine/threonine kinase of ROP2 protein family, plays an important role during invading into host cell, and is an immunosuppressive factor of the host cell-mediated immunity in the stage of parasitophorous vacuole （PV） formation. The knowledge about ROP18 has made great progress with the development of genomics and proteomics. In this paper, as an example of Toxoplasma gondii ROP18, we reviewed the finding history, structure, secretion and allocation, virulence mechanism and application status of ROP18.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2014年第6期645-650,共6页
Chinese Journal of Zoonoses
基金
国家自然科学基金项目(No.31302083)
中央级公益性科研院所基本科研业务费专项资金项目(No.2012JB16
No.2013JB13)
上海市科技兴农重点攻关项目(No.沪农科攻字[2005]第3-4号)联合资助~~
