摘要
目的:通过观察比较低氧耐力运动、单纯低氧暴露和常氧耐力运动4周内大鼠血清、肺组织和主动脉ET-1与NO生成的时序性变化规律,分析有机体对运动与低氧干预的初期应激特征。方法:将120只经过2周适应性训练的SD大鼠分为13组,分别给予中等强度的常氧耐力训练、低氧耐力训练和低氧安静暴露3种干预措施,测定了训练前、训练3、7、14、28天后恢复24h大鼠血清ET-1浓度、肺组织ET-1浓度和NOS(tNOS与iNOS)活性、腹主动脉ET-1和NOS(eNOS与iNOS)mRNA水平。结果:1)常氧运动与低氧运动3天均能够引起血清ET-1水平显著升高(40%~59%,P<0.05),但第7天均回落到训练前水平。低氧安静过程中血清ET-1无显著升高。2)3种干预措施3天均引起肺组织ET-1浓度明显升高(14%~23%,P<0.01),至第14~28天之间均回落至训练前水平。3)常氧运动与低氧运动4周内肺组织不同亚型NOS活性无明显变化,低氧安静第3~7天肺组织tNOS活性开始显著下降(第28天下降幅度为-44%,P<0.01),第14~28天之间iNOS活性开始显著下降(第28天下降幅度为-52%,P<0.01)。4)常氧运动第7~14天之间主动脉ET-1mRNA水平显著升高(115%,P<0.01),随后回落。低氧安静和低氧运动主动脉ET-1mRNA水平则是在开始3天内先显著下降(降幅-80%^-67%,P<0.01),随后逐渐升高,尤以低氧运动引起的升高幅度更为显著,第28天时达到训练前10倍以上(P<0.01),且显著高于其他两种干预措施组(P<0.01)。5)3种干预措施均使大鼠腹主动脉eNOS mRNA水平在3天之内显著升高,随后低氧安静至第3~7天之间以及常氧运动至第14~28天之间开始回落。然而,低氧运动则使腹主动脉eNOS mRNA水平在第14~28天突然大幅度升高,第28天时达到训练前10倍以上(P<0.01),且显著高于其他两种干预措施组(P<0.01)。结论:1)常氧耐力训练初期大鼠血管内皮和肺组织ET-1释放、主动脉ET-1基因表达以及同时期主动脉eNOS基因表达均一过性增�
Purpose： This study aimed to find the traits of stress reaction to hypoxia and moderate intensity endurance training by comparing the time sequence change in endothelin-1 and ni- tric oxide production in organism. Methods：After 2 weeks adaptive training, 120 SD rats were divided into 13 groups and underwent middle intensity normoxic endurance training, hypoxic endurance training and hypoxic sedentary exposure respectively. ET-1 concentration in blood serum and lung, tNOS and iNOS activity in lung, ET- 1, eNOS and iNOS mRNA level in abdominal aorta were measured pre-training, and after 3,7,14 and 28 days of training. Results： 1） Serum ET-1 concentration rise 40%-59% （P〈0.05） after 3 days of normoxic training and hypoxic training, but fell back to pre-training level during 3-7th days of training. Serum ET-1 concentration did not change much during hypoxia sedentary exposure. 2） All the 3 approaches raised ET-1 concentration in lung 14%-23% （P〈0. 01） during 1st-3rd day, which then fell back to pre-training level during 14-28th day. 3） NOS activity in lung didn＇ t change much during the 4 weeks normoxic and hypoxic training. However, after hypoxia sedentary exposure, tNOS activity decreased from 3rd-7th day and to -44% （P〈0.01） at the end of the 4th week, and iNOS activity decreased from 14th- 28th day and to -52 % （P〈 0. 01） at the end of the 4th week. 4） Abdominal aorta ET-1 mRNA level rise 115% （P〈 0. 01）during 7th- 14th day of normoxic training, then fell back. However, it decreased - 80%-67%（P〈0.01） during 1st-3rd day after hypoxic sedentary exposure and hypoxic training, then rise quickly and significantly. At the end of the 4th week, it reached a level more than 10 times of pre-training level （P〈0.01） and was much higher than the other 2 approaches（P〈0. 01）. 5） All of the 3 approaches raised abdominal aorta eNOS mRNA （P〈0.05） within 1-3 days, then fell back after 3-7 days of hypoxic sedentary exposure and 14- 28 days of normoxic training.
出处
《中国体育科技》
CSSCI
北大核心
2014年第4期108-116,共9页
China Sport Science and Technology
基金
国家体育总局体育科学研究所基本科研业务费课题(基本06-20)
关键词
低氧
耐力运动
内皮素1
一氧化氮
时序性变化
低氧习服
鼠
动物实验
Hypoxia
Endurance Training
Endothelin- 1
Nitric Oxide
Time Sequence
Hypoxic Acclimatization
rat
animal experiment
