摘要
本试验通过PCR方法对17株广西猪源猪链球菌2型（SS2）荚膜多糖（CPS）基因cps2J片段进行了扩增、克隆测序，同源性分析结果表明，17株广西SS2的cps2J基因核苷酸和氨基酸的同源性分别在91.9%～99.8%和89.1%～99.7%；菌株可以分为Ⅰ群和Ⅱ群，包括5株发病猪源菌株的16株菌均分在Ⅰ群，Ⅱ群只有GXWX193株。Ⅰ群菌株的CPS基因核苷酸和氨基酸的同源性分别在96.8%～99.8%和94.6%～99.7%，与参考菌（荷兰Netherland、猪源菌株江苏ZYS8和四川SC17、人源菌株江苏98015）的核苷酸和氨基酸的同源性分别在96.4%～99.9%和94.1%～100%。Ⅱ群菌株与参考菌株的核苷酸和氨基酸同源性分别在91.3%～92.4%和89.1%～91.0%。17株广西猪源SS2菌株的cps2J基因序列变异程度小。
Amplified and cloned by PCR, then sequenced the virulence-associated factor cps2J gene of 17 strains of type 2 Streptococcus suis isolated from Guangxi. The homologies of nucleo-tide and predicted amino acids of 17 Guangxi SS2 are 91.9%~99.8% and 89.1%~99.7% respectively.17 SS2 strains could be divided into two groups as Ⅰ （11 strains isolated from healthy pigs and 5 strains from diseased pigs） and Ⅱ （1 strain isolated from healthy pigs）. The homologies of group I were very high （96.8%~99.8% nt and 94.6%~99.7% aa）. Compared to reference strains（Netherland/ZYS8/SC17/98015）, homologies of group Ⅰ were 96.4%~99.9%nt and 94.1%~100%aa , 91.3%~92.4%nt and 89.1%~91.0%aa in group Ⅱ. There is a low degree of variation in cps2J gene in 17 SS2 isolated from pigs in Guangxi ,China.
出处
《现代畜牧兽医》
2014年第6期6-11,共6页
Modern Journal of Animal Husbandry and Veterinary Medicine
基金
广西科技攻关项目(0815009-3-7)广西猪群"高热病"发病
流行和防治模型的研究
关键词
猪链球菌2型
CPS基因
克隆测序
同源性分析
Streptococcus suis type 2
CPS gene
Cloned and sequenced
Homology analysis
