摘要
目的：检测肿瘤坏死因子α（TNF-α）及高危型人乳头瘤病毒（HR-HPV）E2、E6、E7基因在不同宫颈病变组织中的表达并分析其相关性。方法：根据不同病理结果把标本分为HPV阴性正常组，HPV16阳性正常组，HPV16阳性宫颈内瘤变（CIN）Ⅰ～Ⅱ组，HPV16阳性CINⅢ组以及HPV16阳性宫颈腺癌/鳞状细胞癌（CIS/SCC）组。通过反转录聚合酶链反应（RT-PCR）方法对各组标本进行TNF-α、E2、E6及E7蛋白的检测，并对检测结果进行统计学分析。结果：HPV E6、E7基因在不同宫颈病变中的表达逐渐升高，且E7的逐级升高趋势更显著，E2升高至CINⅡ后出现下降，而TNF-α在不同宫颈病变中的表达虽呈升高趋势，但在宫颈浸润癌中与CINⅢ组中表达差异无统计学意义。结论：用E2/E7的比值来预测或诊断高度宫颈病变程度可能更准确。炎症因子TNF-α的升高可能加重了HPV E2基因的破坏，使得HR-HPV E6、E7癌基因过度表达，从而促进宫颈癌发生。
Objective：To detect TNF-α,E2,E6 and E7 in different cervical lesion and analyse the relationship between them. Methods：According to different pathology result, specimens were divided into normal group of HPV negative,HPV16 positive normal group,HPV16 positive CINⅠ-Ⅱ,HPV16 positive CINⅢ,HPV16 positive SCC group. TNF-α,E2,E6 and E7 proteins of each group specimens were detected by using RT-PCR ,and the result of the test were analyzed statistically. Results：HPV E6 and E7 gene expression is positively correlated with the malignant degree of cervical cells ,and the E7 gradually rising trend is more significant. The E2 fall after rise to CINⅡ,and of TNF-αbasic is the rising trend in different cervical lesions ,but the expression has no obvious difference between CINⅢgroup and cervical invasive cancer. Conclusions：With the ratio of E2/E7 to predict or diagnose the degree of high cervical lesions might be more accurate. Elevated inflammatory cytokines of TNF-αmay increase the destruction of E2, which lead to excessive expression of oncogene E6 and E7 ,thus promote the occurrence of cervical cancer.
出处
《国际妇产科学杂志》
CAS
2014年第3期321-323,共3页
Journal of International Obstetrics and Gynecology
关键词
宫颈肿瘤
宫颈上皮内瘤样病变
肿瘤坏死因子α
逆转录聚合酶链反应
乳头状瘤病毒科
Uterine cervical neoplasms
Cervical intraepithelial neoplasia
Tumor necrosis factor-alpha
Reverse transcriptase polymerase chain reaction
Papillomaviridae
